Histochemical study on trematodes - Distribution of carbonic anhydrase activity -

Original Article


Korean J Parasitol. 1972 Apr;10(1):27-33. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1972.10.1.27
Copyright © 1972 by The Korean Society for Parasitology


Histochemical study on trematodes - Distribution of carbonic anhydrase activity -
Jung Kyun Chu,Yong Suk Ryang and You Juang Cho
Department of Parasitology, School of Medicine, Kyung Hee University, Korea.


Abstract
The purpose of the present study is to demonstrate the distribution of carbonic anhydrase pattern in the various termatodes (Fasciola gigantica, Paramphistoma orthocoelium, Paragonimus westermani) by means of Kurada staining method, and to correlate these findings with the histochemical data and harboring location. The results are summarized as follows: 1. In Fasciloa gigantica, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. 2. In Paramphistoma orthocoelium, carbonic anhydrase activity was positive in the vitelline gland cells and eggs in the uterus. 3. In Paragonimus westermani, carbonic anhydrase activity was positive in intestinal mucous membrane, vitelline gland cells and eggs.

Figures


	Fig. 1 Showing the distribution of Carbonic Anhydrase activity in the reticular tissue cells in Fasciola gigantica. (10×40)


	Fig. 2 Showing the distribution of Carbonic Anhydrase activity in vitelline gland cells in Fasciola gigantica. (10×40)


	Fig. 3 Showing the distribution of Carbonic Anhydrase activity in eggs in the uterus in Fasciola gigantica. (10×40)


	Fig. 4 Showing the distribution of Carbonic Anhydrase activity in eggs in the uterus in Paramphistoma orthocoelium. (10×40)


	Fig. 5 Showing the distribution of Carbonic Anhydrase activity at the excretory cannal in Paramphistoma orthocoelium. (10×40)


	Fig. 6 Showing the distribution of Carbonic Anhydrase activity in the vitelline gland cells and intestinal mucous membrane in the Paragonimus westermani. (10×40)


	Fig. 7 Same above. (10×10)

Tables


	Table 1 Distribution of Carbonic Anhydrase in the various trematodes

References


1.	Chu JK, Hong SM. Histochemical studies on Clonorchis sinensis [The distribution of carbonic anhydrase]. Korean J Parasitol 1963;1(1):11–13.

2.	Kurada Y. Stain Tech 1935;28:5–231.