Effects of immunoactivity on <i>Ascaris suum</i> infection in mice

, Rhee, J K; , Park, B K; , Seu, Y S

doi:1991.29.3.279

Korean J Parasito > Volume 29(3):1991 > Article

Original Article


Korean J Parasitol. 1991 Sep;29(3):279-291. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1991.29.3.279
Copyright © 1991 by The Korean Society for Parasitology


Effects of immunoactivity on Ascaris suum infection in mice
J K Rhee,B K Park and Y S Seu
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Korea.


Abstract
The immune response to sheep red blood cell (sRBC) was monitored in the mice infected with Ascaris suum or Trichinella spiralis. The effects of the infection with T. spiralis or the injection with cyclophosphamide(CY) as an immunosuppression agent prior to challenge infection with the embryonated eggs of A. suum were monitored in mice by means of the level of infection with A. suum and cellular and humoral immune response to sRBC. Following the oral administration of 1,000 eggs of A. suum to mice, delayed-type hypersensitivity (DTH) and rosette-forming rate were gradually decreased and reached to the lowest levels at the 5th week and 6th week postinfection, respectively, and then returned to normal at the 10th week. The hemagglutinin(HA) and hemolysin(HE) titers were gradually elevated and reached to peak at the 3rd week postinfection, and then returned to normal level. The appearance ratios of the eosinophils and mast cells were in peak at the 4th week and the 2nd week postinfection, respectively. Meanwhile the harvest ratio of A. suum larvae from the liver and lungs was 21.97% at the 1st week postinfection. Following the oral administration of 300 T. spiralis infective larvae, DTH and rosette-forming rate were gradually decreased with the lapse of time and reached the lowest values in the 30th and 21st day of postinfection, and then slightly increased and transiently decreased in the 70th and 80th day of postinfection, respectively. HA and HE titers were the lowest in the 21st and 90th day, whereas the ratios of eosinophils and mast cells were the highest on the 40th and 14th day postinfection, respectively. Following the intraperitoneal injection of CY, the body weight, the spleen weight, DTH, rosette-forming ratio, HA and HE titers, the number of WBC and the ratio of the mast cell were predominantly decreased in the 5th day, and then returned to the same value of the 1st day postinjection. The ratio of eosinophils was gradually decreased following to advance of days. At the 1st, 5th and 10th days after intraperitoneal injection of CY of 400 mg/kg, a dose with 1,000 eggs of A. suum was administered orally to mice, and harvest rate of the larvae at the 7th day postadministration was 7.07% in the 1st day, 14.94% in the 5th day, 10.1% in the 10th day, 8.02% in control group. The effect of prior infection with infective larvae of T. spiralis upon immunological sequelae of a challenge infection of mice with embryonated eggs of A. suum in 30 or 70 days interval was checked.(ABSTRACT TRUNCATED AT 400 WORDS)

Tables


	Table 1 Effect of age on immunological sequelae to sRBC in mice


	Table 2 Effect of Ascaris suum infection on immunological sequelae to sRBC in mice


	Table 3 Effect of Trichinella spiralis infection on immunological sequelae to sRBC in mice


	Table 4 Effect of cyclophosphamide injection on immunological sequelae in mice


	Table 5 Effect of prior administration with cyclophosphamide upon immunological sequelae and recovery rate following Ascaris suum infection in mice


	Table 6 Dimensions of Ascaris suum larvae detected in a cyclophosphamide infection in mice


	Table 7 Effect of prior infection with Trichinella spiralis upon immunological sequelae of a challenge infection if mice with Ascaris suum


	Table 8 Effect of prior infection with Trichinella spiralis upon larvae recovery of a challenge infection if mice with Ascaris suum


	Table 9 Dimensions of Ascaris suum larvae detected in a challenge infection of prior infection with Trichinella spiralis in mice

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