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Image analytical observation on the growth and development of Clonorchis sinensis in rats
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Korean J Parasito > Volume 33(4):1995 > Article

Original Article
Korean J Parasitol. 1995 Dec;33(4):281-288. English.
Published online Dec 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.4.281
Copyright © 1995 by The Korean Society for Parasitology
Image analytical observation on the growth and development of Clonorchis sinensis in rats
Jin Kim*
Department of Pathology, Chonnam University Medical School, Kwangju 501-190, Korea.
Abstract

For the two-dimensional observation on the growth and development of Clonorchis sinensis, an image analysis system (IBAS 2000, Kontron, Germany) was used in this study. On 3, 6, 9, 12, 15, 21, 30 and 90 days of infection, 474 worms were collected from rats infected with 50 metacercariae each. The overall recovery rate was 31.6%. The worms grew rapidly in their length and width up to 30 days of infection and then, did slowly to 90 days of infection. However, the growth pattern of body area was not similar to that of body length and width, because the body area increased continuously even after 30 days of infection. With the image analysis system, the sizes of irregular-shaped genital organs were measured easily, which showed sigmoidal growth patterns. The growth rate of genital organs increased rapidly until 21 days of infection when the uterus was filled with numerous eggs, and then gradually slowed down to 90 days. There was no difference in growth pattern between the anterior and posterior testis. The seminal receptacle, however, showed an abrupt increase in size between 15 to 21 days of infection when it was filled with condensed semen. Therefore, the growth pattern of seminal receptacle might be used as another criteria to estimate the extent of sexual maturation of C. sinensis. From this study, it is suggested that image analysis system is very useful to reveal the growth and development pattern of C. sinensis, especially of their internal organs.

Figures


Fig. 1
Growth curve of Clonorchis sinensis recovered from the infected rats in various periods of infection


Fig. 2
Growth curve of the genital organs of Clonorchis sinensis recovered from the infected rats in various periods of infection

Tables


Table 1
Results of worm recovery from rats experimentally infected with metacercariae of C. sinensis


Table 2
Distribution of worms in the liver of rats


Table 3
Measurement of C. sinensis recovered from the infected rats in various periods of infection

References
1. Adema CM, Arguello DF 2nd, Stricker SA, Loker ES. A time-lapse study of interactions between Echinostoma paraensei intramolluscan larval stages and adherent hemocytes from Biomphalaria glabrata and Helix aspersa. J Parasitol 1994;80(5):719–727.
  
2. de Diego JA, Higueras A, Gamallo C, Mayer RF, Rey Calero J. [Conventional and analytic morphometry using the IBAS-1 computer in experimental Trypanosoma b. brucei infection]. Ann Parasitol Hum Comp 1987;62(5):387–394.
 
3. Chung DI, Choi DW. Intensity of infection and development of adult Clonorchis sinensis in hamsters. Korean J Parasitol 1988;26(1):9–14.
 
4. Erler BS, Chein K, Marchevsky AM. An image analysis workstation for the pathology laboratory. Mod Pathol 1993;6(5):612–618.
 
5. Hatsushika R, et al. Jpn J Parasitol 1981;30:315–323.
6. Hong SJ, Lee SH, Chai JY, Seo BS. Recovery rate, growth and development of Heterophyopsis continua in experimental chicks. Korean J Parasitol 1990;28(1):53–62.
 
7. Hong ST, Kho WG, Kim WH, Chai JY, Lee SH. Turnover of biliary epithelial cells in Clonorchis sinensis infected rats. Korean J Parasitol 1993;31(2):83–89.
  
8. Kim YI. Liver carcinoma and liver fluke infection. Arzneimittelforschung 1984;34(9B):1121–1126.
 
9. Kobayashi H. Saikingaku Zasshi 1912;20:1–66.
10. Lee WK, Lee KM, Lee OR, Choi WY. [Host-Parasite Relationship In Trematodes: I. Susceptability And Development Of Clonorchis Sinensis In Rabbits. Korean J Parasitol 1973;11(2):76–82.
 
12. Palluault F, Pietrzyk B, Dei-Cas E, Slomianny C, Soulez B, Camus D. Three-dimensional reconstruction of rabbit-derived Pneumocystis carinii from serial-thin sections. I: Trophozoite. J Protozool 1991;38(4):402–407.
 
13. Palluault F, Pietrzyk B, Dei-Cas E, Slomianny C, Soulez B, Camus D. Three-dimensional reconstruction of rabbit-derived Pneumocystis carinii from serial-thin sections. II: Intermediate precyst. J Protozool 1991;38(4):407–411.
 
14. Rhee CH, Seo BS. [The Host-Parasite Relations Of Clonorchis Sinensis In The Mouse: Studies On The Development Of C. Sinensis In Mouse. Korean J Parasitol 1968;6(3):101–109.
 
15. Seo BS. Seoul Univ J Nat Sci 1958;7:1–15.
16. Tasai SS. Jpn J Parasitol 1966;15:246–254.
17. Wykoff DE. Studies on Clonorchis sinensis. III. The host-parasite relations in the rabbit and observations on the relative susceptibility of certain laboratory hosts. J Parasitol 1958;44(5):461–466.
  
18. Yoshimura H, et al. Jpn J Parasitol 1965;14:437–444.
19. Yoshimura H, et al. Jpn J Parasitol 1972;21:222–229.
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