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Autoradiographic studies on the uptake and distribution of 14C-glucose by Paragonimus westermani
Young Ok Park
Department of Parasitology, Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract
Autoradiographic study was performed in order to know the distribution of exogenous 14C-glucose by lung fluke, Paragonimus westermani, incubated in Tyrode medium containing 10 uCi/ml of labeled substance.
After 1 hour incubation at 37℃, microautoradiographs of this fluke showed that black grains derived from radioactive carbon were accumulated mainly in the parenchyme and subcuticular musculature. The muscular tissues such as oral sucker, pharynx and ventral sucker revaled considerable density of fine grains. Slight radioactivity was also observed in the regions of ovary, testes, vitelline follicles, eggs in uterus, intestinal ceca, and even in excretory bladder. Structures showing the least activity included the cuticle and uterine tubules of this fluke.
Figures
Fig. 1 Low density of fine grains were observed in oral sucker, mostly lined near the surface area (×100).
Fig. 2 Low density of fine grains were observed in oral sucker, mostly lined near the surface area (×100).
Fig. 3 The radioactivity was also detected in pharynx, higher than that of oral sucker (×100).
Fig. 4 Black grains were visible in the ventral sucker (×100). Relatively fine grains of them also accumulated near the outer surface.
Fig. 5 High power magnification of intestinal wall revealed that grains were lined along the basement of the intestinal epithelium (×430).
Fig. 6 Conspicuous black grains were observed inside of eggs, in uterus (×430).
Fig. 7 In the region of ovary, slight radioactivity was monitored (×100).
Fig. 9 Microautoradiograhp of testes in which low density of grains were observed (×100).
Fig. 10 High power magnification of the testes (×430).
Fig. 11 High power magnification of vitelline follicles showed slight density of grains (×430).
Fig. 12 Some black grains lined along the wall of excretory bladder in high power magnification (×430).
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