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Dermal mast cell responses in Paragonimus westermani-infected mice
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Korean J Parasito > Volume 35(4):1997 > Article

Original Article
Korean J Parasitol. 1997 Dec;35(4):259-264. English.
Published online Dec 20, 1997.  http://dx.doi.org/10.3347/kjp.1997.35.4.259
Copyright © 1997 by The Korean Society for Parasitology
Dermal mast cell responses in Paragonimus westermani-infected mice
M H Shin*
Department of Parasitology, College of Medicine, Ewha Womans University, Seoul 158-056, Korea.

*Corresponding author (Email: mhshin@mm.ewha.ac.kr)
Received October 04, 1997; Accepted November 20, 1997.

Abstract

This study was carried out to determine whether dermal mast cell responses to Paragonimus westermani in an abnormal host, the mouse, were dependent on the site of metacercarial inoculation. In mice during subcutaneous infection, the number of dermal mast cells were increased significantly (p < 0.05) at the first week (38.3/mm2) and then persisted at a high level until the sixth week (45.2/mm2) of infection compared with PBS-injected (control) mice (range: 19.4-25.1/mm2). In mice during oral infection, the number of dermal mast cells were increased significantly (p < 0.05) at two weeks (33.5/mm2) after infection and remained at these levels thereafter compared with non-infected (control) mice (range: 17.4-22.3/mm2). In mice both during subcutaneous and oral infection, the recruited dermal mast cells showed extensive degranulation at the second week (68.4% and 60.7%, respectively), reached a peak at the third week (81.4%, and 92.1%, respectively) and then declined slightly thereafter. By contrast, in both control mice, about 10% of dermal mast cells were degranulated. In conclusion, this study suggests that dermal mast cell responses to P. westermani in mice are dependent on cutaneous sensitization by larval excretory-secretory antigens, irrespective of infection route.

Figures


Figs. 1-4
Mast cells at the skin in mice during subcutaneous infection with P. westermani compared with PBS-injected (control) mice. Blue spots (arrows) in the dermis and subcutaneous layer are the mast cells. 1. PBS-injected (control) mice, showing only small number of dermal mast cells, ×100. 2. In mice during subcutaneous infection, week 1 PI, showing a significant increase in the number of mast cells of dermis and subcutaneous layer, ×100. 3. In mice during subcutaneous infection, week 2 PI, many mast cells are distributed adjacent to a cyst of worm, ×100. 4. In mice during subcutaneous infection, week 3 PI, degranulated mast cells with open-faced nuclei ard shown around a cyst of worm in subcutaneous layer, ×200. Toluidine blue staining, pH 0.5.


Figs. 5-6
In mice during subcutaneous (5) and oral infection (6) with P. westermani, respectively, week 3 PI, many mast cells are degranulated, ×100. A rectangle indicates higher magnification (×200) of fully extensive degranulated mast cells. The nucleus of mast cell is heavily stained and numerous cytoplamic granules are extruded from the cell. Toluidine blue staining, pH 0.5.

Tables


Table 1
Dermal mast cell number and extent of mast cell degranulation during P. westermani infection in BALB/c mice

References
1. Arizono N, Kasugai T, Yamada M, Okada M, Morimoto M, Tei H, Newlands GF, Miller HR, Kitamura Y. Infection of Nippostrongylus brasiliensis induces development of mucosal-type but not connective tissue-type mast cells in genetically mast cell-deficient Ws/Ws rats. Blood 1993;81(10):2572–2578.
 
2. Boctor FN, Peter JB. IgG subclasses in human chronic schistosomiasis: over-production of schistosome-specific and non-specific IgG4. Clin Exp Immunol 1990;82(3):574–578.
  
3. Chai JY, Kim TH, Kho WG, Chung SW, Hong ST, Lee SH. Mucosal mast cell responses to experimental Metagonimus yokogawai infection in rats. Korean J Parasitol 1993;31(2):129–134.
  
4. Chernin J, Miller HR, Newlands GF, McLaren DJ. Proteinase phenotypes and fixation properties of rat mast cells in parasitic lesions caused by Mesocestoides corti: selective and site-specific recruitment of mast cell subsets. Parasite Immunol 1988;10(4):433–442.
  
5. Cutts L, Wilson RA. Elimination of a primary schistosome infection from rats coincides with elevated IgE titres and mast cell degranulation. Parasite Immunol 1997;19(2):91–102.
  
6. Falus A, Meretey K. Histamine: an early messenger in inflammatory and immune reactions. Immunol Today 1992;13(5):154–156.
  
7. Im KI, Hwang HK, Soh CT. [Behaviour Of Mast Cells In Mice In The Course Of Entamoeba Histolytica Infection By Strains]. Korean J Parasitol 1975;13(2):115–122.
 
8. Kho WG, et al. Seoul J Med 1990;31:191–199.
9. Lindsay MC, et al. Int J Parasitol 1985;15:201–209.
 
10. Miller HR, Newlands GF, McKellar A, Inglis L, Coulson PS, Wilson RA. Hepatic recruitment of mast cells occurs in rats but not mice infected with Schistosoma mansoni. Parasite Immunol 1994;16(3):145–155.
  
11. Mimori T, Nawa Y, Korenaga M, Tada I. Strongyloides ratti: mast cell and goblet cell responses in the small intestine of infected rats. Exp Parasitol 1982;54(3):366–370.
  
12. Min DY, Ryu JS, Shin MH. Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani. Korean J Parasitol 1993;31(3):231–238.
  
13. Romagnani S. Induction of TH1 and TH2 responses: a key role for the natural immune response?. Immunol Today 1992;13(10):379–381.
  
14. Rousseaux-Prevost R, Capron M, Bazin H, Capron A. IgE in experimental schistosomiasis. II. Quantitative determination of specific IgE antibodies against S. mansoni: a follow-up study of two strains of infected rats. Correlation with protective immunity. Immunology 1978;35(1):33–39.
 
15. Shin MH, Min DY. Production of interferon-gamma and interleukin-4 by splenocytes in mice infected with Paragonimus westermani. Korean J Parasitol 1996;34(3):185–189.
  
16. Shin MH, Min HK. Effects of anti-IgE mAb on serum IgE, Fc epsilon RII/CD23 expression on splenic B cells and worm burden in mice infected with Paragonimus westermani. Korean J Parasitol 1997;35(1):47–54.
  
17. Watanabe N, Nawa Y, Okamoto K, Kobayashi A. Expulsion of Hymenolepis nana from mice with congenital deficiencies of IgE production or of mast cell development. Parasite Immunol 1994;16(3):137–144.
  
18. Weinstock JV, Boros DL. Modulation of granulomatous hypersensitivity VI T lymphocyte subsets influence mast cell density in liver granulomas of Schistosoma mansoni-infected mice. J Immunol 1983;131(2):959–961.
 
19. Woodbury RG, Miller HR, Huntley JF, Newlands GF, Palliser AC, Wakelin D. Mucosal mast cells are functionally active during spontaneous expulsion of intestinal nematode infections in rat. Nature 1984;312(5993):450–452.
  
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