Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Attempts to transfer immunity against Clonorchis sinensis in nude and DS mice
| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasito Search

CLOSE

Korean J Parasito > Volume 29(4):1991 > Article

Original Article
Korean J Parasitol. 1991 Dec;29(4):371-380. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.4.371
Copyright © 1991 by The Korean Society for Parasitology
Attempts to transfer immunity against Clonorchis sinensis in nude and DS mice
D W Choi,D I Chung and D H Kang
Department of Parasitology, Kyungpook National University, School of Medicine, Taegu 700-422, Korea.
Abstract

The effects of peritoneal exudate cells (PEC) and sera of athymic nude and DS mice infected with Clonorchis sinensis metacercariae or sensitized by injection of metabolic products into footpad on transfer of immunity against the fluke to the syngeneic mice were studied.

There was no significant difference in eggs per gram pattern between the sensitized and control groups, and between nude and DS mice. However, the worm burdens were slightly greater in nude mice than in DS mice. Also, a few plaque forming cells were found in only DS mice given PEC and serum from Group II DS mice.

In the light of these results, it is likely that PEC and sera of nude or DS mice which are deficient, at least partially, in the cellular immune system are unable to transfer immunity against C. sinensis to syngeneic recipients.

Tables


Table 1
Experimental groups to study the effects of peritoneal exudate cells and sera on transfer of immunity against Clonorchis sinensis in nude and DS mice


Table 2
Proportion of viable cells per ml of peritoneal exudate cells in donor nude and DS mice by trypan blue exclusion


Table 3
Effect of sensitized peritoneal exudate cells and sera on Eggs per Gram of feces in recipient nude and DS mice after challenging infection


Table 4
Effect of peritoneal exudate cells and sera sensitized by oral infection or footpad injection of metabolic products of adult C. sinensis on worm burdens C. sinensis in recipient nude and DS mice


Table 5
Viable spleen cells and plaque forming cells in recipient mice after challenging infection

References
1. Cate CC. A successful method for exsanguinating unanesthetized mice. Lab Anim Care 1969;19(2):256–258.
 
2. Cho WH, et al. Keimyung Univ Med J 1988;7:193–203.
3. Choi DW, et al. Kyungpook Univ Med J 1987;28:135–142.
4. Choi DW, et al. Kyungpook Univ Med J 1990;31:39–49.
5. Choi DW, et al. Kyungpook Univ Med J 1985;26:302–311.
6. Choi DW, et al. Kyungpook Univ Med J 1987;28:194–202.
7. Choi DW, et al. Kyungpook Univ Med J 1990;31:8–16.
8. Choi DW, et al. Kyungpook Univ Med J 1986;27:146–152.
9. Choi DW, et al. Kyungpook Univ Med J 1989;30:301–311.
10. Choi DW, et al. Kyungpook Univ Med J 1987;28:263–273.
11. Colley DG. Schistosomal egg antigen-induced lymphocyte blastogenesis in experimental murine Schistosoma mansoni infection. J Immunol 1971;107(5):1477–1480.
 
12. Cypess R. Demonstration of immunity to Nematospiroides dubius in recipient mice given spleen cells. J Parasitol 1970;56(1):199–200.
  
13. Dodd K, Nuallain TO. Effect of antilymphocytic sera on the histopathology of Fasciola hepatica infestation in rabbits. J Pathol 1969;99(4):335–337.
  
14. Flanagan SP. 'Nude', a new hairless gene with pleiotropic effects in the mouse. Genet Res 1966;8(3):295–309.
  
15. Fuji H, Zaleski M, Milgrom F. Immune response to alloantigens of thymus studied in mice with plaque assay. J Immunol 1971;106(1):56–64.
 
16. Hsu CK, Hsu SH, Whitney RA Jr, Hansen CT. Immunopathology of schistosomiasis in athymic mice. Nature 1976;262(5567):397–399.
  
17. Isaak DD, Jacobson RH, Reed ND. Thymus dependence of tapeworm (Hymenolepis diminuta) elimination from mice. Infect Immun 1975;12(6):1478–1479.
 
18. Jacobson RH, Reed ND. The thymus dependency of resistance to pinworm infection in mice. J Parasitol 1974;60(6):976–979.
  
19. Jerne NK, Nordin AA. Plaque Formation in Agar by Single Antibody-Producing Cells. Science 1963;140(3565):405.
  
20. Kitamura Y, Okamoto S, Uchida N, Yamaguchi K, Matsumoto K. Effect of androgen depletion on growth and androgen dependency of Shionogi carcinoma 115. Cancer Res 1978;38(12):4711–4716.
 
21. Kwon TC, Kang CM, Choi DW. [Passive transfer of immunity against Clonorchis sinensis by peritoneal exudate cells in mice]. Korean J Parasitol 1987;25(1):45–50.
 
22. Lang BZ, Larsh JE Jr, Weatherly NF, Goulson HT. Demonstration of immunity to Fasciola hepatica in recipient mice given peritoneal exudate cells. J Parasitol 1967;53(1):208–209.
  
23. Larsh JE, et al. J Mitchell Sci Soc 1964;80:133–135.
24. Larsh JE Jr, Race GJ, Goulson HT, Weatherly NF. Studies on delayed (cellular) hypersensitivity in mice infected with Trichinella spiralis. 3. Serologic and histopathologic findings in recipients given peritoneal exudate cells. J Parasitol 1966;52(1):146–156.
  
25. Lim TJ, et al. Keimyung Univ Med J 1987;6:167–176.
26. Pantelouris EM. Absence of thymus in a mouse mutant. Nature 1968;217(5126):370–371.
  
27. Phillips SM, DiConza JJ, Gold JA, Reid WA. Schistosomiasis in the congenitally athymic (nude) mouse. I. Thymic dependency of eosinophilia, granuloma formation, and host morbidity. J Immunol 1977;118(2):594–599.
 
28. Rajasekariah GR, Mitchell GF, Chapman CB, Montague PE. Fasciola hepatica: attempts to induce protection against infection in rats and mice by injection of excretory/secretory products of immature worms. Parasitology 1979;79(3):393–400.
  
29. Ritchie LS. An ether sedimentation technique for routine stool examinations. Bull U S Army Med Dep 1948;8(4):326.
 
30. Stoll NR. Am J Hyg 1923;3:59–70.
31. Sun T. The in vitro action of antisera on the adults of Clonorchis sinensis. Trans R Soc Trop Med Hyg 1969;63(5):582–590.
  
32. Tamura T, Ueda K, Furuta T, Goto Y, Fujiwara K. Electron microscopy of spontaneous pneumocystosis in a nude mouse. Jpn J Exp Med 1978;48(4):363–368.
 
33. Ueda K, Goto Y, Yamazaki S, Fujiwara K. Chronic fatal pneumocystosis in nude mice. Jpn J Exp Med 1977;47(6):475–482.
 
34. Walzer PD, Schnelle V, Armstrong D, Rosen PP. Nude mouse: a new experimental model for Pneumocystis carinii infection. Science 1977;197(4299):177–179.
  
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2024 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI