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Korean J Parasitol > Volume 21(2):1983 > Article

Original Article
Korean J Parasitol. 1983 Dec;21(2):270-280. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1983.21.2.270
Copyright © 1983 by The Korean Society for Parasitology
Comparison of agar-gel diffusion tests, counterimmunoelectrophoresis and enzyme-linked immunosorbent assay in the sera of skin test positives for paragonimiasis
Ok-Ran Lee and Won-Young Choi
Department of Parasitology, College of Medicine, Soonchunhyang University, Choong-Nam, Korea.
Department of Parasitology, Catholic Medical College and Catholic Institute of Parasitic Diseases, Seoul, Korea.

Agar-gel diffusion test (AGD), counterimmunoelectrophoresis (CIEP) and enzyme-linked immunosorbent assay(ELISA) were examined with the sera of skin test positives for paragonimiasis.

The crude antigen(Paragonimus whole worm extracts: protein concentration, 7.56mg/ml) and human sera were used in AGD and CIEP. And in ELISA test, diluted antigen with 1:40,000 of crude antigen and diluted sera with 1:100, 1:200 were used in the test.

The positive identical ratio between AGD and CIEP reactions is 98% and negative identical ratio is 100%. One or three precipitin bands are observed in AGD. One to seven precipitin bands are also revealed in CIEP. Especially, deeply stained bands are observed in CIEP than those of AGD.

The positive identical ratios between AGD and ELISA tests are 96% in 1:100 diluted sera, and 94% in 1:200 diluted sera. But the negative identical ratios between AGD and ELISA tests are 97% and 99% respectively in 1:100 and 1:200 diluted sera.

The positive identical ratios between CIEP and ELISA tests are 98% and 96% respectively in 1:100 and 1:200 diluted sera, but also 97% and 99% in 1:100 and 1:200.

Control sera, such as clonorchiasis, amoebiasis and toxoplasmosis, revealed all negatives with Paragonimus antigen in AGD, CIEP and ELISA tests.

By above results, ELISA was most sensitive, next CIEP and AGD. But AGD test appears to be more useful when used to crude antigen without cross reaction with other parasitic infections. CIEP test is basically equal in terms of precipitin reaction, but CIEP is able to be detected more sensitively and rapidly though less simple in handiwork than AGD.

Consequently, three methods for immunological tests of paragonimiasis have good correlations with one another. Also, each of these has both merits and demerits in immunological test for paragonimiasis. But the ELISA test was proved to be the most sensitive and convenient tool for mass screening test, especially in case of using purified antigen.


Fig. 1
OD-distribution in ELISA test of 155 sera for Paragonimus skin test positive persons.

Fig. 2
Precipitin bands in AGD test.

Center wells: Paragonimus antigen, Outer wells: No. of sera, Table 1: No. 4, 6, 8, 15, 23, 43, Table 2: No. 1, 11, 49, 55, 93

Fig. 3
Precipitin bands in AGD and CIEP.

Center wells: Paragonimus antigen, Outer wells: No. of sera, Table 1: No. 2, 24, Table2: No. 68, 75

Fig. 4
Precipitin bands in AGD and CIEP.

Center wells: Paragonimus antigen, Outer wells: No. of sera, Table 1: No. 32, 37, 46, 47, Table 2: No. 92

Fig. 5
Comparison of ELISA reaction for sera of clonorchiasis, amoebiasis, toxoplasmosis and Paragonimus skin test positive with Paragonimus antigen.


Table 1
Results of CIEP and ELISA reaction in 52 sera of AGD positive persons

Table 2
Results of CIEP and ELISA reaction in 103 sera of AGD negative persons

Table 3
OD-distribution in different serum dilution of ELISA reaction in positives and negatives by AGD reaction

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