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Korean J Parasitol > Volume 28(4):1990 > Article

Original Article
Korean J Parasitol. 1990 Dec;28(4):207-212. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.4.207
Copyright © 1990 by The Korean Society for Parasitology
ELISA of rat sera infected with Paragonimus iloktsuenensis
B K Lim,O R Lee and H S Nam
Department of Parasitology, College of Medicine, Soonchunhyang University, Chun-An 300-090, Korea.

Enzyme-linked immunosorbent assay (ELISA) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of Paragonimus iloktsuenensis (PIA), P. westermani (PWA) and Clonorchis sinensis (CSA). Three crude antigens (PIA, PWA, CSA) were prepared to saline homogenated supernatants of whole adult worms. Infected rat sera were obtained biweekly from the albino rats fed 50-80 metacercariae of P. iloktsuenensis through gastric catheter. Experimental groups were divided into 4 groups: GI (controls), GII, GIII and GIV according to 1-7 worms as GII, 10-19 worms as GIII and 22-40 worms as GIV, respectively.

In ELISA, the mean OD values of each group for the homologous antigen (PIA) were increased significantly compared to the control sera at the 4th week of infection. With the progress of duration of infection, the mean OD values of infected sera of GII & GIV continuously increased up to the 12th week (last week), but in GIII the mean OD value increased until the 10th week. No significance was noted among the infection dose groups (GII, GIII and GIV), after the 6th week of infection. Also, the OD values of all infected rats did not show any proportional relationships to the number of worms recovered. In brief, the antibody productivity of individual rats were strongly different. The rat sera infected with P. iloktsuenensis cross-reacted with those infected with P. westermani or C. sinensis, as identified by OD values.


Fig. 1
The mean OD values in ELISA of each group for Paragonimus iloktsuenensis antigen and rat sera.

Fig. 2
The mean OD values in ELISA of Paragonimus iloktsuenensis rat sera and each antigen of PIA, PWA or CSA.


Table 1
Recovered worms of Paragonimus iloktsuenensis in each rat group

Table 2
The mean OD values in ELISA of paragonimiasis iloktsuenensis rat sera (100× diluted) and crude antigens; P. iloktsuenensis (PIA), P. westermani (PWA), and C. sinensis (CSA)

Table 3
Anova & Duncan's multiple comparison test for the OD values of rat sera infected with P. iloktsuenensis (value of Pi replaced by rank)

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