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Korean J Parasitol > Volume 23(2):1985 > Article

Original Article
Korean J Parasitol. 1985 Dec;23(2):285-292. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1985.23.2.285
Copyright © 1985 by The Korean Society for Parasitology
Evaluation of Giardia lamblia detection method in stool specimens fixed with SAF solution
Young Chang Kim,Jae Jin Kim and Keun Tae Lee
Department of Parasitology, Yonsei University College of Medicine, Korea.
Abstract

The present study has been designed as a basic study on laboratory diagnosis of giardiasis and to demonstrate a more effective method for the detection of Giardia lamblia cyst with the inherent advantages of minimizing both the number of stool examinations required and the interval of stool collections for estimating the real state of prevalence in the shortest time possible. There were 3 subject groups of 75 children each currently residing in an orphanage in Gunsan city, Jeonbuk province from which stool specimens were collected every day, every other day, and every 3 days. The procedure is as follows: Resuspend the fixed sample after fixation with SAF solution. Centrifuge the sediment for 1 min. at 2,000 rpm after straining through gauze into a tube. Divide the sediment into 3 parts and use them for direct fecal smear, formalin-ether concentration (MGL) and zinc sulfate (ZnSO(4)) floatation techniques. The results are summarized as follows: Overall infection rate after 10 trials showed a 60 percent positive indication. The positive rate among children under 4 years old was significantly higher than the rate in children over 4 years old. No significant difference in rate by sex was observed. The results of examinations by direct fecal smear and MGL techniques appeared more accurate than that obtained by ZnSO(4) floatation method as indicated by a higher positive rate. Of all three methods concerned, combinations of two demonstrated a higher positive rate than that shown by any one alone. In three consecutive examinations under varying conditions such as different days, the cyst detection rate by MGL technique indicated 83 percent. In 5 examinations under the same varying conditions, the indicated rate was 94 percent. The interval of stool collection proved to be insignificant for the cyst detection rate. In conclusion, both MGL method and modified fecal direct smear can provide a good cyst detection rate of G. lamblia provided that more than 3 consecutive examinations of stool under varying conditions are carried out.

Figures


Fig. 1
Comparison of the recovery rate in n examinations between practical and expected proportions

*remarks p.r.: practical rate e.r.: expected rate

▥ 1st exam. ▤ 2nd exam. ▨ 3rd exam. ▧ 4th exam. ■ 5th exam.


Tables


Table 1
Prevalence of Giardia lamblia by sex and age


Table 2
Observed efficiencies of individual techniques and of their combinations in detecting G. lamblia cysts from fecal specimens


Table 3
Results in detecting G. lamblia cysts from feces by techniques; direct smear technique, MGL sedimentation technique, ZnSO4 floatation technique


Table 4
True efficiencies in n examinations by means of techniques; direct smear, MGL sedimentation and ZnSO4 techniques


Table 5
Expected recovery rates of G. lamblia cyst from feces utilizing individual technique and their combinations


Table 6
Recovery rates of G. lamblia cysts by number of MGL sedimentation technique

References
1. Notis WM. Giardiasis and vitamin B 12 malabsorption. Gastroenterology 1972;63(6):1085.
 
2. Ament ME, Rubin CE. Relation of giardiasis to abnormal intestinal structure and function in gastrointestinal immunodeficiency syndromes. Gastroenterology 1972;62(2):216–226.
 
3. Antia FP, Desai HG, Jeejeebhoy KN, Kane MP, Borkar AV. Giardiasis in adults. Incidence symptomatology and absorption studies. Indian J Med Sci 1966;20(7):471–477.
 
4. Bemrick WJ. Observations on dogs infected with giardia. J Parasitol 1963;49:1031–1032.
  
5. Blagg W, Schloegel EL, Mansour NS, Khalaf GI. A new concentration technic for the demonstration of protozoa and helminth eggs in feces. Am J Trop Med Hyg 1955;4(1):23–28.
 
6. Brady PG, Wolfe JC. Waterborne giardiasis. Ann Intern Med 1974;81(4):498–499.
 
7. Brodsky RE, Spencer HC Jr, Schultz MG. Giardiasis in American travelers to the Soviet Union. J Infect Dis 1974;130(3):319–323.
  
8. Brooke MM, et al. J Lab Clin Med 1949;34:1554–1560.
9. Garrocho-Sandoval C, Torres-Ruvalcaba A. Diagnosis of intestinal parasitic infestation. Study of two methods for collection of specimens. Am J Clin Pathol 1977;67(6):603–606.
 
10. Cho KM, et al. Yonsei Rep Trop Med 1973;4:50–58.
11. Chyu I, et al. These of Catholic Med College 1965;9:159–172.
12. Cortner JA. Giardiasis, a cause of celiac syndrome. AMA J Dis Child 1959;98:311–316.
 
13. Craun GF. Waterborne giardiasis in the United States: a review. Am J Public Health 1979;69(8):817–819.
  
14. Danciger M, Lopez M. Numbers of Giardia in the feces of infected children. Am J Trop Med Hyg 1975;24(2):237–242.
 
15. Faust EC. Am J Trop Med 1930;10:137–138.
16. Faust EC, et al. Am J Trop Med 1938;18:169–189.
17. Faust EC, et al. J Parasit 1939;25:241–262.
 
18. Guinn E. The use of the zinc sulfate centrifugation flotation technic as a routine diagnostic procedure. Am J Clin Pathol 1959;31(1):87–88.
 
19. Jeffery GM. A three-year epidemiologic study of intestinal parasites in a selected group of mental patients. Am J Hyg 1960;71:1–8.
 
20. Jeffery GM, Harrison AJ. Intestinal parasitism in a group of mental patients during seven years of observation. J Parasitol 1963;49:308–312.
 
21. Kamath KR, Murugasu R. A comparative study of four methods for detecting Giardia lamblia in children with diarrheal disease and malabsorption. Gastroenterology 1974;66(1):16–21.
 
22. Keystone JS, Krajden S, Warren MR. Person-to-person transmission of Giardia lamblia in day-care nurseries. Can Med Assoc J 1978;119(3):241–242.
 
23. Kim CH, Park CH, Kim HJ, Chun HB, Min HK, Koh TY, Soh CT. [Prevalence Of Intestinal Parasites In Korea]. Korean J Parasitol 1971;9(1):25–38.
 
24. Kim SC, Kim JJ, Lee KT. [Epidemiological Studies On Protozoan Infection In Gyeong-Gi Do And Jeonra Bug Do]. Korean J Parasitol 1984;22(1):116–126.
 
25. Min HK. [Studies on the incidence of the intestinal protopozoa in the mountainous area of Chollapuk-do]. Korean J Parasitol 1972;10(1):8–21.
 
26. Moore GT, Cross WM, McGuire D, Mollohan CS, Gleason NN, Healy GR, Newton LH. Epidemic giardiasis at a ski resort. N Engl J Med 1969;281(8):402–407.
  
27. Notis WM. Giardiasis and vitamin B 12 malabsorption. Gastroenterology 1972;63(6):1085.
 
28. Giardiasis: manifestations and diagnosis. JAMA 1977;237(11):1078–1079.
29. Petersen H. Scand J Gastroenterol 1972;7(14) Suppl:1–44.
30. Ritchie LS. Bull US Army Med Dept 1948;8:326.
31. Ritchie LS, et al. J Parasit 1952;38:16.
32. Sapero JJ, Lawless DK. The MIF stain-preservation technic for the identification of intestinal protozoa. Am J Trop Med Hyg 1953;2(4):613–619.
 
33. Sawitz WG, et al. Am J Trop Med 1942;22:130–136.
34. Scholten T. An improved technique for the recovery of intestinal protozoa. J Parasitol 1972;58(3):633–634.
  
35. Scholten TH, Yang J. Evaluation of unpreserved and preserved stools for the detection and identification of intestinal parasites. Am J Clin Pathol 1974;62(4):563–567.
 
36. Soh CT, et al. Yonsei Med J 1961;2:31–41.
37. Svensson R, et al. Acta Medica Scandinavia 1934;81:267–324.
 
38. Tsuchiya H. Am J Hyg 1931;13:544–567.
39. Willis HH. Med J Australia 1921;8:375–376.
40. Wright SG. Giardiasis and malabsorption. Trans R Soc Trop Med Hyg 1980;74(4):436–437.
  
41. Wright SG, Tomkins AM, Ridley DS. Giardiasis: clinical and therapeutic aspects. Gut 1977;18(5):343–350.
  
42. Yang J, Scholten T. A fixative for intestinal parasites permitting the use of concentration and permanent staining procedures. Am J Clin Pathol 1977;67(3):300–304.
 
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