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Korean J Parasitol > Volume 23(2):1985 > Article

Original Article
Korean J Parasitol. 1985 Dec;23(2):247-252. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1985.23.2.247
Copyright © 1985 by The Korean Society for Parasitology
Studies on the comparative migration patterns of Ascaris suum larvae between primary and re-infected mice
Jong Sool Song,Jae Jin Kim,Duk Young Min and Keun Tae Lee
Department of Parasitology, Yonsei University College of Medicine, Korea.

In the present study, the effect of primary infection to reinfection with Ascaris suum larvae was experimented in mouse model. Mice were challenged with l,000 infective stage eggs of Ascaris suum. The embryonated eggs were directly introduced into stomach of mice. Reinfection was performed at 50 days after the primary infection with same method as primary infection. Mice were sacrificed 3, 5, 7, 10, 15 and 20 days after infection in both groups respectively. Larvae collected from livers and lungs with Baermann's apparatus were enumerated and measured after sacrifice. Sera of mice were also collected at same time. The results of the experiment were as follows: With antigen prepared from coelomic fluid of adult Ascaris suum and sera collected from mice before reinfection, the production of antibody in experimental mice was confirmed by the gel-diffusion technique. In the livers of reinfected mice, the larvae were recovered up to 10 days after challenge, otherwhile in the primary infected mice, the larvae were observed up to 7 days. The maximum number of larvae were observed in the lungs of primary infected mice on 10 days after inoculation. In the lungs of reinfected mice, maximum number of larvae were recovered on 7 days after, only few larvae were recovered on 10 days after reinfection. As regards the growth of the larvae, the third stage larvae, over 500 µm in length, appeared in livers at 5 days after reinfection, but it couldn't be found on 7 days and 10 days after challenge. The third stage larvae continuously developed were observed in lungs of mice from 5 days after reinfection. In conclusion, it was found that development of larvae in livers of immune mice were probably repressed by the immune mechanisms being rised in livers and defence mechanism is also acting by interfering with the process of larval penetration into the lung from the liver.


Fig. 1
Distribution of larvae detected from lungs in mice infected with Ascaris suum.

Fig. 2
Distrivution larvae detected from livers in mice infected with Ascaris suum.

Fig. 3
Average length of Ascaris suum larvae recovered from livers and lungs in infected mice.

Fig. 4
Average width of Ascaris suum larvae recovered from livers and lungs in infected mice.


Table 1
Distribution of larvae recovered from livers and lungs at various intervals in mice infected with Ascaris suum eggs

Table 2
Lengths of larvae recovered from livers and lungs of mice following oral infection with 1,000 Ascaris suum eggs.

1. Bindseil E. Immunity to Ascaris suum. 1. Immunity induced in mice by means of material from adult worms. Acta Pathol Microbiol Scand 1969;77(2):218–222.
2. Bindseil E. Immunity to Ascaris suum. 2. Investigations of the fate of larvae in immune and non-immune mice. Acta Pathol Microbiol Scand 1969;77(2):223–234.
3. Bindseil E. Immunity to Ascaris suum. 3. The importance of the gut for immunity in mice. Acta Pathol Microbiol Scand B Microbiol Immunol 1970;78(2):183–190.
4. Crandall CA, Arean VM. The protective effect of viable and nonviable Ascaris suum larvae and egg preparations in mice. Am J Trop Med Hyg 1965;14(5):765–769.
5. FALLIS AM. Ascaris lumbricoides infection in guinea pigs with special reference to eosinophilia and resistance. Can J Res 1948;26(5):307–327.
6. Jenkins DC. Observations on the early migration of the larvae of Ascaris suum Goeze, 1782 in white mice. Parasitology 1968;58(2):431–440.
7. Jeska EL, Williams JF, Cox DF. Ascaris suum: larval returns in rabbits, guinea pigs and mice after low-dose exposure to eggs. Exp Parasitol 1969;26(2):187–192.
8. Johnstone C, Leventhal R, Soulsby EJ. The spin method for recovering tissue larvae and its use in evaluating C57BL/6 mice as a model for the study of resistance to infection with Ascaris suum. J Parasitol 1978;64(6):1015–1020.
9. Kelley GW, Nayak DP. Acquired immunity to migrating larvae of ascaris suum induced in pigs by repeated oral inoculations in infective eggs. J Parasitol 1964;50:499–503.
10. Kerr KB. Am J Hyg 1938;27:28–51.
11. Martin HM. Res Neb Agric Exp Stn 1926;37:1–78.
12. Schenkel RH. Diss Abs Int 1972;32B:6131.
13. Soulsby EJ. Immunization against Ascaris lumbricoides in the guinea pig. Nature 1957;179(4563):783–784.
14. Soulsby EJ. J Am Vet Med Ass 1961;138:355–362.
16. Stromberg BE, Soulsby EJ. Ascaris suum: immunization with soluble antigens in the guinea pig. Int J Parasitol 1977;7(4):287–291.
17. SPRENT JF, CHEN HH. Immunological studies in mice infected with the larvae of Ascaris lumbricoides; criteria of immunity and immunizing effect of isolated worm tissues. J Infect Dis 1949;84(2):111–124.
18. Taffs LF. Immunological studies on experimental infection of pigs with Ascaris suum Goeze, 1782. VI. The histopathology of the liver and lung. J Helminthol 1968;42(1):157–172.
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