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Korean J Parasitol > Volume 23(2):1985 > Article

Original Article
Korean J Parasitol. 1985 Dec;23(2):247-252. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1985.23.2.247
Copyright © 1985 by The Korean Society for Parasitology
Studies on the comparative migration patterns of Ascaris suum larvae between primary and re-infected mice
Jong Sool Song,Jae Jin Kim,Duk Young Min and Keun Tae Lee
Department of Parasitology, Yonsei University College of Medicine, Korea.
Abstract

In the present study, the effect of primary infection to reinfection with Ascaris suum larvae was experimented in mouse model. Mice were challenged with l,000 infective stage eggs of Ascaris suum. The embryonated eggs were directly introduced into stomach of mice. Reinfection was performed at 50 days after the primary infection with same method as primary infection. Mice were sacrificed 3, 5, 7, 10, 15 and 20 days after infection in both groups respectively. Larvae collected from livers and lungs with Baermann's apparatus were enumerated and measured after sacrifice. Sera of mice were also collected at same time. The results of the experiment were as follows: With antigen prepared from coelomic fluid of adult Ascaris suum and sera collected from mice before reinfection, the production of antibody in experimental mice was confirmed by the gel-diffusion technique. In the livers of reinfected mice, the larvae were recovered up to 10 days after challenge, otherwhile in the primary infected mice, the larvae were observed up to 7 days. The maximum number of larvae were observed in the lungs of primary infected mice on 10 days after inoculation. In the lungs of reinfected mice, maximum number of larvae were recovered on 7 days after, only few larvae were recovered on 10 days after reinfection. As regards the growth of the larvae, the third stage larvae, over 500 µm in length, appeared in livers at 5 days after reinfection, but it couldn't be found on 7 days and 10 days after challenge. The third stage larvae continuously developed were observed in lungs of mice from 5 days after reinfection. In conclusion, it was found that development of larvae in livers of immune mice were probably repressed by the immune mechanisms being rised in livers and defence mechanism is also acting by interfering with the process of larval penetration into the lung from the liver.

Figures


Fig. 1
Distribution of larvae detected from lungs in mice infected with Ascaris suum.


Fig. 2
Distrivution larvae detected from livers in mice infected with Ascaris suum.


Fig. 3
Average length of Ascaris suum larvae recovered from livers and lungs in infected mice.


Fig. 4
Average width of Ascaris suum larvae recovered from livers and lungs in infected mice.

Tables


Table 1
Distribution of larvae recovered from livers and lungs at various intervals in mice infected with Ascaris suum eggs


Table 2
Lengths of larvae recovered from livers and lungs of mice following oral infection with 1,000 Ascaris suum eggs.

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