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Korean J Parasitol > Volume 23(1):1985 > Article

Original Article
Korean J Parasitol. 1985 Jun;23(1):165-172. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1985.23.1.165
Copyright © 1985 by The Korean Society for Parasitology
Effect of prednisolone treatment on the experimental inducement of primary amoebic meningoencephalitis
Ok Yong Kim,Kyung Il Im,Keun Tae Lee and Rim Soon Choe
Department of Parasitology, College of Medicine, Yonsei University, Seoul, Korea.
Department of Biology, College of Science, Yonsei University, Korea.

Present study aimed to elucidate the immunosuppressive effect of prednisolone on Naegleria fowleri infection in mice. N. fowleri was cultured in CGVS medium (Willert and Le Ray, 1973). White female mice, weighing about 18 g, used for experiments were divided into five groups; untreated control group, prednisolone treated groups (before, during and after infection), and only prednisolone treated group. In the prednisolone treated group, the hormone was injected intramuscularly 5 doses of 10 mg/kg every other day. According to designated time of treatment, each mouse was challenged with 1 × 10(5) N. fowleri intranasally. Changes of body weights, clinical manifestations and number of dead mouse were observed. Brain and lung tissues of dead mice were cultured in the non-nutrient agar (Kasprzak and Mazur, 1972), or stained with hematoxylin-eosin for the examination of histopathological changes. Results of the experiment are summarized as follows: Mortality among the prednisolone treated groups was higher than that in untreated control group, and among the treated groups, the pretreated group showed shorter survival time. Body weights among untreated control mice showed no significant increase, however, treated groups of mice showed the decrease during the administration and recovery of the weights were observed at 2 to 3 days after the completion of treatment. In the treated control groups, the infected mice began to show the pathologic findings 5 days after infection while the untreated mice began to show the findings 8 days after infection. Tissue damages in brain and lung occurred due to virulence of amoeba were more severe among treated mice than that in untreated control group. The above mentioned results suggest that the treatment with prednisolone weaken the resistance of mice against N. fowleri infection, and probably induce more severe primary amoebic meningoencephalitis. Especially severe pathological findings were shown in pre-treated group, compared with untreated group.


Fig. 1
Cumulative death rate of mice due to N. fowleri infection by post-infection day.

Fig. 2
Changes in weights of mice during the experimental periods. Arrow head (▼) indicates the day of infection in each group except Group 4.

Figs. 3-7
Fig. 3. Mouse brain of the right is infected with N. fowleri, showing edema, hemorrhage and necrosis, and that of the left is normal.

Fig. 4. Mouse brain infected with N. fowleri, showing marked inflammatory reaction and necrotic lesion (H-E stain, ×40).

Fig. 5. Higher magnification of Fig. 4. Trophozoites (arrows) are well distinguished (H-E, ×400).

Fig. 6. Mouse lung of the right is infected with N. fowleri, showing congestion, and that of the left is normal.

Fig. 7. Mouse lung infected with N. fowleri, showing inflammatory reaction and hemorrhage. Trophozoites (arrows) are distinguished (H-E, ×400).


Table 1
Cumulative number of dead mouse dut to N. fowleri infection in each experimental groups

Table 2
Observation of major clinical manifestations in infected mice

Table 3
Pathologic findings in mice infected with N. fowleri.

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