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Korean J Parasitol > Volume 22(2):1984 > Article

Original Article
Korean J Parasitol. 1984 Dec;22(2):229-237. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1984.22.2.229
Copyright © 1984 by The Korean Society for Parasitology
Migration and distribution of spargana in body of experimentally infected mice
Won Jin Choi
Department of Surgery, College of Medicine, Seoul National University, Seoul 110, Korea.
Abstract

The migration and distribution pattern of spargana in mouse body was observed after experimental infection through mouth. The spargana were obtained from the snake, Natrix tigrina lateralis, caught in Hoengseong-gun, Kangwon-do. A total of 28 male mice (ICR strain), 21-25g in body weight, were fed each with 5 scolices (and necks) of spargana and killed after 10 minutes to 14 days. Systemic autopsy was performed on each mouse to recover the spargana.

The results are as follows:

1. The spargana were found to penetrate into the stomach or duodenal wall of mice as early as 10 minutes after infection. They completed the penetration within 30 minutes and appeared in abdominal cavity. It was observed that spargana did not migrate tangentially along the gut wall but directly perforated the wall.

2. After 1 hour to 1 day the majority of spargana distributed in abdominal cavity of mice except few which migrated to muscles or subcutaneous tissues.

3. It was within 7 days that nearly all of the spargana migrated to subcutaneous tissues. Out of total 28 in number found from subcutaneous tissues, 13 distributed around neck region, 12 around trunk and other 3 on head of mice and the most common sites were submandibular and subscapular areas. There was nearly no host tissue reaction to migrating spargana.

4. The initial length of spargana given was 4 mm in average but it increased to 12 mm after 7 days and to 35 mm after 14 days.

The results suggest that spargana orally given to mice penetrate the gut wall within 30 minutes followed by escaping into abdominal cavity, and after passing through thoracic cavity or abdominal wall they finally localize in subcutaneous tissues chiefly around neck region within 7 days.

Figures


Figs. 1-7
Fig. 1. Three spargana collected from the subcutaneous tissue of a snake, Unnecessary posterior portions had been cut off form easy experimental infection to mice.

Fig. 2. Eight spargana recovered 7 days after infection from subcutaneous tissues of mice. Note th enlarged size.

Fig. 3. Nine spargana recovered 14 days after infection from subcutaneous tissues of mice. Their size enlarged more.

Fig. 4. a sparganum(arrow) penetration the duodenal wall and escaping into the abdominal cavity, 10 minutes after infection.

Fig. 5. Two spargana (arrows) escaping from the duedenum, 10 minutes after infection.

Fig. 6. Mucosal (A) and serosal (B) views of mouse duodenum showing three spargana having penetrated into the wall, 20 minutes after infection. Direct perforation of the wall is suggestive in this figure.

Fig. 7. A sparganum (arrow) migrating on the mesentery adjacent to jejunum of a mouse, 30 minutes after infection.

*All scales in Fig. 1-7 are given in mm.



Fig. 8-11
Fig. 8. Cross section of a mouse duodenum penetrated by a sparganum, 10 minutes after infection. Direct perforation of the wall is evident in this figure. ×40.

Fig. 9. Section of another sparganum penetration the duodenal wall, 20 minutes after infection. ×40.

Fig. 10. Another sparganum penetration the duodenal wall 2 hours after infection. The serosa is relatively intact and this indicates that the site of perforation by sparganum may by very small, ×40.

Fig. 11.Ibid. The mucosal layer of mouse duodenum showing pressure atrophy by the penetrating sparganum. x100.

**Abbreviations: SC-scolex of sparganum, N-neck, IN-intestine of mouse.


Tables


Table 1
Recovery of spargana from mice after oral infection


Table 2
Extra-intestinal locations of spargana after experimental infection in mice


Table 3
Subcutaneous (or muscular) locations of spargana after experimental infection in mice

References
1. Anders K, Foley K, Stern E, Brown WJ. Intracranial sparganosis: an uncommon infection. Case report. J Neurosurg 1984;60(6):1282–1286.
  
2. Chi JG, Chi HS, Lee SH. Histopathologic Study On Human Sparganosis. Korean J Parasitol 1980;18(1):15–23.
 
3. Iwata S. Progress of Medical Parasitology in Japan 1972;4:536–590.
4. Kim SC, et al. J Korean Neurosurgery Assoc 1981;10:589–593.
5. Kobayashi E. Taiwan Igakkai Zasshi 1931;30(4):363–380.
6. Kobayashi E. Taiwan Igakkai Zasshi 1931;30(2):16–40.
7. Lee SH, Chai JY, Seo BS, Cho SY. Two cases of human infection by adult of Spirometra erinacei. Korean J Parasitol 1984;22(1):66–71.
 
8. Maietsu M. Jikken Igaku Zasshi 1928;12:232–247.
9. Mineura K, Mori T. Sparganosis of the brain. Case report. J Neurosurg 1980;52(4):588–590.
  
10. Mueller JF. Am J Trop Med 1938;18(3):303–328.
11. Mueller JF. Am J Trop Med 1938;18(1):41–66.
12. Mueller JF. The laboratory propagation of Spirometra mansonoides as an experimental tool. V Behavior of the sparganum in and out of the mouse host, and formation of immune precipitates. J Parasitol 1961;47:879–883.
  
13. Mueller JF. The biology of Spirometra. J Parasitol 1974;60(1):3–14.
 
14. Mueller JF, et al. J Parasit 1963;49(2):294–296.
 
15. Swartzwelder JC, Beaver PC, Hood MW. Sparganosis in Southern United States. Am J Trop Med Hyg 1964;13:43–47.
 
16. Yokogawa S, et al. Trans 8th Congress Far East Assoc. Top Med 1930;2:215–226.
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