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Korean J Parasitol > Volume 21(2):1983 > Article

Original Article
Korean J Parasitol. 1983 Dec;21(2):265-269. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1983.21.2.265
Copyright © 1983 by The Korean Society for Parasitology
Comparison of TIA with ELISA for circulating antibody detection in clonorchiasis
Yong-Kie Lee,Jae-Sook Ryu,Keun-Tae Lee and Kyung-Il Im*
Department of Parasitology, College of Medicine, Yonsei University, Korea.
*Department of Parasitology, College of Medicine, Hanyang University, Korea.
Abstract

A comparison was made of a new serological method, thin layer immunoassay (TIA), and an established method, enzyme-linked immunosorbent assay (ELISA), in the detection and quantification of antibodies in clonorchiasis.

Saline extract of lyophilized Clonorchis sinensis adult worm was used as antigen, and TIA by the method of Elwing et al. (1976) and ELISA by Voller et al. (1974) were performed. Using sera from known clonorchiasis cases, 100 percent of the sera tested were positive by TIA and 88.3 percent by ELISA. TIA produced false positive results in 14 out of 36 cases, which were 10 amoebiasis cases, 16 paragonimiasis cases and 10 healthy controls. ELISA, however, produced a small number of false positives, 7 out of 55 cases.

There was correlation between immunoglobulin G level in sera and ELISA value (correlation coefficient, 0.69), whereas no correlation between immunoglobulin G level and TIA result. The performance of TIA and ELISA was not correlated in the results using homologous antigen.

Figures


Fig. 1
Relationship between Immunoglobulin G level and TIA zone diameter in sera from clonorchiasis patients.


Fig. 2
Relationship between Immunoglobulin G level and ELISA value in sera from clonorchiasis patients.


Fig. 3
Scatter diagram showing the relation between the extinction of ELISA and the zone diameter of TIA using clonorchiasis sera.


Fig. 4
The relationship between ELISA value and wheal size obtained with intradermal test in clonorchiasis patients.

Tables


Table 1
Results of TIA in 10 healthy control, 60 clonorchiasis, 10 amoebiasis and 16 paragonimiasis patients


Table 2
Results of ELISA in 24 healthy control, 60 clonorchiasis, 20 amoebiasis and 11 toxoplasmosis patients

References
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7. Sadun EH, Walton BC, Buck AA, Lee BK. The use of purified antigens in the diagnosis of clonorchiasis sinensis by means of intradermal and complement fixation tests. J Parasitol 1959;45(2):129–134.
  
8. Sawada T, Takei K, Williams JE, Moose JW. Isolation and purification of antigen from adult Clonorchis sinensis for complement fixation and precipitin tests. Exp Parasitol 1965;17(3):340–349.
  
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10. Voller A, et al. Bull Wld Hlth Org 1974;51:209.
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