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Korean J Parasitol > Volume 17(2):1979 > Article

Original Article
Korean J Parasitol. 1979 Dec;17(2):147-153. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1979.17.2.147
Copyright © 1979 by The Korean Society for Parasitology
Immunoelectrophoretic studies of several helminths
Won-Young Choi and Ok-Ran Lee
Department of Parasitology, Catholic Medical College, Korea.

In an attempt to investigate the antigen-antibody relations and the value of immunodiagnosis for several helminths, Ouchterlony tests and immunoelectrophoreses were carried out. Taenia saginata, Cysticercus sp. of cestodes, Clonorchis sinensis, Fasciola hepatica and Paramphistomum sp. of Trematodes,and Ascaris suum of nematodes were used as antigens.

On the other hand, antisera were obtained by injecting 0.5 ml each of the above antigens and the same amount of complete Freund's adjuvant into rabbits ten times at an interval of one week. The result obtained in this study are as follows:

1. A larger number of precipitin arcs were demonstrated in homologous antigen-antibody reactions than in heterologous antigen-antibody reactions both in Ouchterlony tests and immunoelectrophoreses.

2. Gross reactions were observed between the different species of the same class, but no cross reactions were noticed when the classes were different with one or two exceptions, such as between T. saginata, F. hepatica and A. suum.

3. In A. suum, the difference between the male and female was more distinct in Ouchterlony test and immunoelectrophoresis than in the examination of organs such as genital organ and coeliac fluid.

4. Immunoelectrophoresis revealed specific arcs and higher sensitive reaction than Ouchterlony test, and was considered to be a more valuable method for identifing species and immunological diagnosis.


Figs. 1-8
Immunoelectrophoresis between parasitic antigens and anti-parasitic rabbit sera showing the patterns of crossreactions.

1. Upper trough (=UT): Anti-Cysticercus serum, Central well (=CW): Antigen of Taenia saginata, Lower trough(=LT): Anti-T. saginata serum.

2. UT: Anti-Fasciola hepatica serum, CW: Antigen of Taenia saginata, LT: Anti-T. saginate serum.

3. UT: Anti-Paramphistomum serum, CW: Antigen of Taenia saginata, LT: Anti-T. saginate serum.

4. UT: Anti-Clonorchis sinensis serum, CW: Antigen of Taenia saginata, LT: Anti-T. saginate serum.

5. UT: Anti-Paragonimus westermani serum, CW: Antigen of Taenia saginata, LT: Anti-T. saginate serum.

6. UT: Anti-Ascaris suum (♀) serum, CW: Antigen of Taenia saginata, LT: Anti-T. saginate serum.

7. UT: Anti-Cysticercus serum, CW: Antigen of Cysticercus sp., LT: Anti-T. saginate serum.

8. UT: Anti-Paramphistomum serum, CW: Antigen of Paramphistomum sp., LT: Anti-Paragonimus westermani serum.


Table 1
Precipitin reactions among several helminths in Ouchterlony tests

1. Choi WY, Kimura K, Tsuji M. [Immunoelectrophoresis For Anthelmintics Evaluation Against Experimental Paragonimiasis]. Korean J Parasitol 1976;14(2):94–102.
2. Choi WY, Lee OR. [Immunoelectrophoresis for Fasciola hepatica]. Korean J Parasitol 1979;17(1):73–80.
3. Han SH, Woo CK. [Immuno-Diffusion And Complement-Fixation Test In Paragonimiasis Of Cat]. Korean J Parasitol 1969;7(3):160–165.
4. Kagan IG, Norman L. Analysis of Helminth Antigens (Echinococcus Granulosus and Schistosoma Mansoni) by Agar Gel Methods. Ann N Y Acad Sci 1963;113:130–153.
5. Kent NH. Comparative Immunochemistry of Larval and Adult from of Schistosoma Mansoni. Ann N Y Acad Sci 1963;113:100–113.
6. Tada I. Physiological and serological studies of Paragonimus miyazakii infection in rats. J Parasitol 1967;53(2):292–297.
7. Yogore MG, et al. Am J Trop Med & Hyg 1965;14:586–591.
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