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Korean J Parasitol > Volume 17(2):1979 > Article

Original Article
Korean J Parasitol. 1979 Dec;17(2):121-126. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1979.17.2.121
Copyright © 1979 by The Korean Society for Parasitology
The wormicidal substances of fresh water fishes on Clonorchis sinensis I Preliminary research on the wormicidal substance from mucous substance of Carassius carassius
Jae Ku Rhee,Byeong Kirl Baek,Byung Zun Ahn and Young Jun Park
Department of Veterinary Medicine, College of Agriculture, Jeonnam National University, Korea.
Korea Research Institute of Chemical Technology, Korea.
Department of Veterinary Medicine, College of Agriculture, Jeonbug National University, Korea.
Abstract

The present work was to observe the wormicidal effects of the external mucous substance of Carassius carassius and Pseudorasbora parva on cercaria, metacercaria (liberating) and adult of Clonorchis sinensis. The mucous substance which was extracted by ether was separated into various spots on the silica gel thin layer chromatography and separated into various fractions in the silica gel column chromatography, using petroleum ether(30%) and chloroform(70%) as solvent.

1. Rf 0.952 value of the silica gel thin layer chromatography and the first fraction (reddish yellow) frorn the silica gel column chromatography of the ether extracts from C. carassius had the wormicidal effect on all of the developing stages of C. sinensis. Cercaria was killed within 14 minutes, metacercaria within 27 minutes and adult 2.5 hours by the first fraction.

2. But in the normal saline solution (control) and in the other fraction, cercaria and metacercaria were alive more than 24 hours and adult more than 72 hours.

3. The extracts from the mucous substance of P. parva that is well penetrated by cercaria had no wormicidal effect on them.

Figures


Fig. 1
The cercaria of Clonorchis sinensis obtained from Parafossarulus manchouricus, ×100.


Fig. 2
The separated tail of the cercaria observed with in 2 minutes in the wormicidal fraction from the mucous substance of Carassius carassius, ×100.


Fig. 3
The folded metacercaria living more than 24 hours in the control, ×100.


Fig. 4
The killed metacercaria in the wormicidal fraction from C. carassius in 27 minutes, ×200.


Fig. 5
The contents in the intestine of the adult continued to appear for more than 72 hours in the control, ×100.


Fig. 6
The contents in the intestine was defecated after about 2.5 hours in the wormicidal fraction of C. carassius, ×100.


Fig. 7
The posterior intestine of C. carassius contained the intestinal material for more than 72 hours in the control, ×100.


Fig. 8
The material in the posterior intestine was discharged after about 2.5 hours in the wormicidal fraction of C. carassius, ×100.

Tables


Table 1
Wormicidal effects of ether extracted fractions of the mucous substance from C. carassius on the cercaria of C. sinensis


Table 2
Wormicidal effects of ether extracted fractions of the mucous substance from C. carassius on the metacercaria of C. sinensis


Table 3
Wormicidal effects of ether extracted fractions of the mucous substance from C. carassius on the adult of C. sinensis

References
1. Chun SK. [Studies On The Experimental Mode Of Infections Of Clonorchis Sinensis: II. Experimental Infection Of Clonorchis Sinensis Cercaria To Fry Of Fresh Water Fishes]. Korean J Parasitol 1964;2(3):137–147.
 
2. Chun SK. [Studies on the experimental mode of infections of Clonorchis sinensis: III. Studies on the wormicidal effect of external mucous substance of some fresh water fish on the larva of Clonorchis sinensis]. Korean J Parasitol 1964;2(3):148–158.
 
3. Rhee JK. [Experimental studies on the second intermediate hosts of Clonorchis sinensis: III. Observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of Clonorchis sinensis in fresh-water fish]. Korean J Parasitol 1974;12(2):101–110.
 
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