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Korean J Parasitol > Volume 9(1):1971 > Article

Original Article
Korean J Parasitol. 1971 Apr;9(1):17-24. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1971.9.1.17
Copyright © 1971 by The Korean Society for Parasitology
Autoradiographic studies on the uptake of 14C-succinic acid by Clonorchis sinensis
Seung Soo Han
Department of Internal Medicine, college of Medicine, Seoul National University, Korea.
Abstract

The distribution of exogenous 14C-succinic acid by Clonorchis sinensis incubated in Tyrode medium was studied by using autoradiographic techniques. A comparison was made between macro and microautoradiographs of this fluke.

After 1 hour incubation, microautoradiographs of this worm showed that black grains derived from labeled substance were distinctly observed in parenchymal tissue, oral sucker, ventral sucker, ovary, testes, and uterine tubules with eggs. In pharynx and intestine, a low density was monitored while negligible activity was found in seminal receptacle and vitelline follicles.

In studying the further details, microautoradiography was utilized. The most radioactive regions were the reticular tissue and subcuticular musculature of this fluke, closely compatible with the finding of macroautoradiography. Apparent black grains of radioactivity were also found in oral sucker, ventral sucker, pharynx, uterine tubules, eggs in uterus, and intestinal ceca. Structures showing the least radioactivity included ovary, seminal receptacle and vitelline folliciles.

Figures


Fig. 1
Macroautoradiographiy of Clonorchis sinensis incubated in Tyrode solution with 14C-succinic acid for 1 hour.


Fig. 2
Microautoradiograph of C. sinensis showing high concentration of radioactivity in parenchymal tissue (×100).


Fig. 3
High power magnification of parenchymal tissue (×430). Black grains were congregated in subcuticular and reticular tissue.


Fig. 4
Low concentration of fine grains were observed in oral sucker, and congregated particles appeared in pharynx (×100).


Fig. 5
High power magnification of Fig. 4 (×403).


Fig. 6
Distinct black grains were visible in ventral sucker (×100).


Fig. 7
The uterine convolution and eggs in uterus. Note the high density of grains in the eggs and its surrounding uterine tubules (×100).


Fig. 8
High power magnification of Fig. 7 (×430). Dark grains were mostly located inside the egg.


Fig. 9
Conspicuous particles lined along the intestinal wall (×430).


Fig. 10
In the region of testis, grains accumulated densely (×100).


Fig. 11
Grains were hardly observed in vitelline follicles (×100).


Fig. 12
Some particles accumulated in patch, in the region of ovary. But no grain was seen inside of seminal receptacle (×100).

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