| Home | E-Submission | Sitemap | Contact us |  
Korean J Parasitol > Volume 9(1):1971 > Article

Original Article
Korean J Parasitol. 1971 Apr;9(1):1-7. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1971.9.1.1
Copyright © 1971 by The Korean Society for Parasitology
Hatching and activation of some cestode ova - The effects of various artificial hatching-activating solutions upon the some cestode ova -
Seung Chull Park
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.

The effects of various digestive enzymes, fresh biles, and bile acids on the hatching and activation of the eggs of Taenia saginata, T. pisiformis, T. solium, Hymenolepis nana, and Moniezia expansa have been investigated. On the basis of the results of the present study author attempted to elucidate the specificity of host-parasite relationships in the tapeworm infections.

The results were summarized as follows:

1) In case of the ova of T. saginata, pretreatment with artificial gastric juice before contact of intestinal juice is required to bring the disintegration of the embryophore and activation of the embryo. However the ova of T. solium and T. pisiformis may be disintegrated and activated directly into the artificial intestinal juice without the above pretreatment. Among the digestive enzymes applied in this experiment, trypsin was found most effective on the hatching.

2) No hatching of the ova of H. nana and M. expansa was observed in the above artificial hatching-activating solutions.

3) The fresh biles and bile salts were more effective on the hatching and activation of Taeniid ova than sodium taurocholate and sodium deoxycholate.

4) It is turned out that the digestive enzymes or bile acids in the intermediate hosts may, not apparently be essential factor of determining the specificity of host-parasite relationships.


Fig. 1
Diagrammatic section of the taeniid egg.

Explanation of Figures
Sequence of hatching and activation of Taeniid ova. (×970)

Fig. 2. A mature taeniid egg surrounded by yolk substance and chorionic membrane.

Fig. 3. Taeniid ova with intact embryophere.

Fig. 4. Darkening and swelling of embryophore.

Fig. 5. Blakening of embryophore.

Fig. 6. A disintegration embryophore showing rod-shaped blocks.

Fig. 7. Wedge-shaped embryophoric blocks.

Fig. 8. The embryophore is disintegrating and releasing the onchosphere with its membrane.

Fig. 9. Hatched onchosphere freed from their enclosing embryophore.

Fig. 10. Showing the activated hexacanth embryo as the hooks begin to move to rupture the enclosing onchospheral membrane.

Fig. 11. (a) Hatched non-activate onchosphere, (b) Activated onchosphere, (c) Previously activated but dead onchosphere note the randomly arranged hooklets.


Table 1
Comparative effects of various hatching solutions upon the cestode ova

Table 2
Comparative effects of various hatching-activating solutions upon the Taenia saginata (1), Taenia solium (2) and Taenia pisiformis (3)

1. Berntzen AK, Voge M. In Vitro Hatching Of Oncospheres Of Four Hymenolepidid Cestodes. J Parasitol 1965;51:235–242.
2. Isobe M. J Med Ass Formosa 1922;22:161–178.
3. Jones AW, Segarra JM, Wyant KD. Growth and hatching of taeniid eggs. J Parasitol 1960;46:170–174.
4. Di Conza JJ. Hatching requirements of dwarf tapeworm eggs (Hymenolepis nana) in relation to extraintestinal development of larval stages in mice. Z Parasitenkd 1968;31(3):276–281.
5. Laws GF. Chemical ovacidal measures as applied to Taenia hydatigena, Taenia ovis, Taenia pisiformis, and Echinococcus granulosus. Exp Parasitol 1967;20(1):27–37.
6. Laws GF. The hatching of taeniid eggs. Exp Parasitol 1968;23(1):1–10.
7. Meyers HF. Physical observations on Echinococcus eggs. II. A simple method for the determination of viability. J Parasitol 1957;43(3):322–323.
8. Meymarian E. Host-parasite relationships in echinococcosis. VI. Hatching and activation of Echinococcus granulosus ova in vitro. Am J Trop Med Hyg 1961;10:719–726.
9. Morseth DJ. Ultrastructure Of Developing Taeniid Embryophores And Associated Structures. Exp Parasitol 1965;16:207–216.
10. Ogren RE. Morphology and development of oncospheres of the cestode Oochoristica symmetrica Baylis, 1927. J Parasitol 1957;43(5):505–520.
11. Ogren RE. The hexacanth embryo of a dilepidid tapeworm. III. The formation of shell and inner capsule around the oncosphere. J Parasitol 1959;45:580–585.
12. Silverman PH. Studies on the biology of some tapeworms of the genus Taenia. I. Factors affecting hatching and activation of taenid ova, and some criteria of their viability. Ann Trop Med Parasitol 1954;48(2):207–215.
13. Silverman PH. Studies on the biology of some tapeworms of the genus Taenia. II. The morphology and development of the Taeniid hexacanth embryo and its enclosing membranes, with some notes on the state of development and propagation of gravid segment. Ann Trop Med Parasitol 1954;48(4):356–366.
14. Voge M, Berntzen AK. In vitro hatching of oncospheres of Hymenolepis diminuta (Cestoda: Cyclophyllidea). J Parasitol 1961;47:813–818.
15. Voge M, Edmonds H. Hatching in vitro of oncospheres from coracidia of Lacistorhynchus tenuis (Cestoda: Tetrarhynchidea). J Parasitol 1969;55(3):571–573.
16. Webbe G. Z Tropenmed Parasit 1967;81:354–369.
17. Yoshino K. J Med Ass Formosa 1933;32:139–141.
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
Editorial Office
c/o Department of Medical Environmental Biology
Chung-AngUniversity College of Medicine, Dongjak-gu, Seoul 06974, Korea
Tel: +82-2-820-5683   Fax: +82-2-826-1123   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2021 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI