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Korean J Parasitol > Volume 9(1):1971 > Article

Original Article
Korean J Parasitol. 1971 Apr;9(1):1-7. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1971.9.1.1
Copyright © 1971 by The Korean Society for Parasitology
Hatching and activation of some cestode ova - The effects of various artificial hatching-activating solutions upon the some cestode ova -
Seung Chull Park
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract

The effects of various digestive enzymes, fresh biles, and bile acids on the hatching and activation of the eggs of Taenia saginata, T. pisiformis, T. solium, Hymenolepis nana, and Moniezia expansa have been investigated. On the basis of the results of the present study author attempted to elucidate the specificity of host-parasite relationships in the tapeworm infections.

The results were summarized as follows:

1) In case of the ova of T. saginata, pretreatment with artificial gastric juice before contact of intestinal juice is required to bring the disintegration of the embryophore and activation of the embryo. However the ova of T. solium and T. pisiformis may be disintegrated and activated directly into the artificial intestinal juice without the above pretreatment. Among the digestive enzymes applied in this experiment, trypsin was found most effective on the hatching.

2) No hatching of the ova of H. nana and M. expansa was observed in the above artificial hatching-activating solutions.

3) The fresh biles and bile salts were more effective on the hatching and activation of Taeniid ova than sodium taurocholate and sodium deoxycholate.

4) It is turned out that the digestive enzymes or bile acids in the intermediate hosts may, not apparently be essential factor of determining the specificity of host-parasite relationships.

Figures


Fig. 1
Diagrammatic section of the taeniid egg.


Explanation of Figures
Sequence of hatching and activation of Taeniid ova. (×970)

Fig. 2. A mature taeniid egg surrounded by yolk substance and chorionic membrane.

Fig. 3. Taeniid ova with intact embryophere.

Fig. 4. Darkening and swelling of embryophore.

Fig. 5. Blakening of embryophore.

Fig. 6. A disintegration embryophore showing rod-shaped blocks.

Fig. 7. Wedge-shaped embryophoric blocks.

Fig. 8. The embryophore is disintegrating and releasing the onchosphere with its membrane.

Fig. 9. Hatched onchosphere freed from their enclosing embryophore.

Fig. 10. Showing the activated hexacanth embryo as the hooks begin to move to rupture the enclosing onchospheral membrane.

Fig. 11. (a) Hatched non-activate onchosphere, (b) Activated onchosphere, (c) Previously activated but dead onchosphere note the randomly arranged hooklets.


Tables


Table 1
Comparative effects of various hatching solutions upon the cestode ova


Table 2
Comparative effects of various hatching-activating solutions upon the Taenia saginata (1), Taenia solium (2) and Taenia pisiformis (3)

References
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