| Home | E-Submission | Sitemap | Contact us |  
top_img
Korean J Parasitol > Volume 7(3):1969 > Article

Original Article
Korean J Parasitol. 1969 Dec;7(3):160-165. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1969.7.3.160
Copyright © 1969 by The Korean Society for Parasitology
Immuno-diffusion and complement-fixation test in paragonimiasis of cat
Sang Hee Han and Chang Kyu Woo
Department of Parasitology, Catholic Medical College, Seoul, Korea.
Abstract

In order to observe the complement fixation test and immuno-diffusion test of paragonimiasis, the sera taken at 10 days intervals up to 150 days from cats infected with Paragonimus westermani were examined by the above two immunological methods.

The resultant findings were as follows:

1) The complement fixation test showed positive reaction 20 days after the infection with 20 metacercariae, and 40-50 days after the infection with 10 metacercariae. The highest titer was observed 110 days later following the acceleration at 80 days later.

2) In immuno-diffusion test, one are appeared 30 days after the infection with 20 metacercariae, but 60 days after the infection with 10 metacercariae. However, more than two arcs were observed since 70 days after infection.

3) A relatively wide band appeared by the antigens of Fresh worm material and Somatic material. But relatively clear precipitin lines were observed in the diffusion test with V.B.S. antigen, increasing to 3-4 arcs after 110days.

4) In general, complement fixation test showed earlier and higher sensitive reaction than immuno-diffusion test, and was considered to be more valuable method forr immunological diagnosis.

Figures


Fig. 1
Serial chahge of C.F. titer in cats infected by P. westermani.


Plate 1
Center; Serum of cat No. 2, 130 days after infection with 10 m.c.

1. F.W.M. 10×

2. F.W.M. 20×

3. F.W.M. 30×

4. F.W.M. 40×

5. F.W.M. 50×

6. F.W.M. 60×

Wide bands are seen by No. 1 and No. 2 wells and relatively clear two arcs by No. 3 and No. 4 wells but only arc by No. 6 well.



Plate 2
Center; Serum of cat No. 4, 130 days after infection with 10 m.c.

1. V.B.S. 100×

2. V.B.S. 200×

3. V.B.S. 300×

4. V.B.S. 400×

5. V.B.S. 500×

6. V.B.S. 1,000×

Clear precipitin lines were found by every wells and 4 arcs by the 100 times antigen. (No. 1 well)



Plate 3
Center; Serum of cat No. 7, 60 days after transplantation.

1. V.B.S. 100×

2. V.B.S. 200×

3. V.B.S. 300×

4. F.W.M. 10×

5. F.W.M. 20×

6. F.W.M. 30×

Two arcs are seen by No. 1, No. 2 and No. 3 wells and relatively wide bands by No. 4, No.5 and No. 6 wells.



Plate 4
Diagrammatic; illustration of Plate 2.

Tables


Table 1
Antibosies in Cat's sera appeared in I.D. test and C.F. test (m.c. ……metacercaria of P. westermaani)


Table 2
Agar diffusion arcs appeared by different antigen and duration after the infection with 10 metacercariae.


Table 3
Days of the first appearance and acceeleration of Complement fixation test and agar diffusion test.

References
1. Ando R. Jpn J Microbiol Soc 1921;15:391–404.
2. Chyu I. Kor J Int Med 1962;5:37–45.
3. Chyu I, et al. J Cath Med Coll 1965;9:153–158.
4. Chyu I, et al. Bull NIH 1961;4:88–93.
5. Hunter GW 3rd, Ritchie LS, Pan C, Lin S, Sugiura S, Nagano K, Yokogawa M. Immunological studies, II. Intradermal tests and their application in the field for the detection of schistosomiasis japonica, paragonimiasis and clonorchiasis. Mil Med 1958;122(2):85–96.
 
6. Katsuhiko Y, et al. Jpn J Hyg 1959;14:104–107.
7. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
 
8. Ouchterlony O. Diffusion-in-gel methods for immunological analysis. Prog Allergy 1958;5:1–78.
 
9. Sadun EH, Buck AA, Walton BC. The diagnosis of paragonimiasis westermani using purified antigens in intradermal and complement fixation tests. Mil Med 1959;124(3):187–195.
 
10. Seed JR, et al. J Parasit 1966;52:358–362.
 
11. Seed JR, et al. Tulane Stud Zool Bot 1968;15:70–74.
12. So N. Fukuoka Acta Med 1954;50:2594–2623.
13. Tada I. Physiological and serological studies of Paragonimus miyazakii infection in rats. J Parasitol 1967;53(2):292–297.
  
14. Yogore MG, et al. Am J Trop Med Hyg 1965;14:586–591.
15. Yoo JS, et al. Kor Cent J Med 1966;10:351–365.
Editorial Office
c/o Department of Medical Environmental Biology
Chung-AngUniversity College of Medicine, Dongjak-gu, Seoul 06974, Korea
Tel: +82-2-820-5683   Fax: +82-2-826-1123   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2021 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI