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Korean J Parasitol > Volume 2(3):1964 > Article

Original Article
Korean J Parasitol. 1964 Dec;2(3):165-169. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1964.2.3.165
Copyright © 1964 by The Korean Society for Parasitology
Autoradiographic studies on some parasitic helminth
Joo Soo Yoon,Soon Hyung Lee and Byong Seol Seo
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.

Direct contact method of autoradiography was utilized in studying the distribution of exogenous C14-proline in Clonorchis sinensis, Eurytrema pancreaticum, Hymenolepis diminuta and Dipylidium caninum. The most distinct autoradiogram were obtained after 60 min of incubation, corresponding to the maximal absorption of C14-proline in these parasite. The radioactivitity of this labeled amino acid was chiefly concentrated in reproductive organs, especially egg-containing uterine tubules.


Fig. 1
The autoradiograms of Clonorchis sinensis incubated in Tyrode solution with C14-labeled proline. The most distinct radioactivity was shown in uterine tubules.

Fig. 2
The autoradiogram of Eurytrema pancreaticun. C14-labeled proline was most strikingly concentrated in ovary and testes. High degree of radioactivity was also observed in uterine tubules.

Fig. 3
The autoradiogram of Hymenolepis diminuta. The most radioactive regions were the vitelline gland and the testes, in mature proglottids.

Fig. 4
The autoradiograms of Dipylidium caninum. The most striking densities were seen in the ovary, vitelline gland, testes and egg capsules of the mature proglottids. In the egg capsules of gravid segments, the conspicuous tracer activity was also notes.

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