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Korean J Parasitol > Volume 36(1):1998 > Article

Original Article
Korean J Parasitol. 1998 Mar;36(1):37-45. English.
Published online Mar 20, 1998.  http://dx.doi.org/10.3347/kjp.1998.36.1.37
Copyright © 1998 by The Korean Society for Parasitology
A Clonorchis sinensis-specific antigen that detects active human clonorchiasis
S I Kim
Department of Parasitology, Chosun University College of Medicine, Kwangju 501-759, Korea.
Received November 24, 1997; Accepted January 21, 1998.

Abstract

A Clonorchis sinensis-specific antigen in excretory-secretory product of C. sinensis (CsE) was assessed in human clonorchiasis by immunoblot. Thirty and 7 kDa antigens of CsE2, one of four different batches of CsEs reacted strongly with infection sera from clonorchiasis patients; however, the antigens reacted weakly with 6-month post-treatment sera from praziquantel-cured cases, but were still highly detected by the sera from praziquantel-failed patients, indicating that the 30 and 7 kDa antigens can detect antibodies during an active infection. The 30 kDa antigen showed some cross reactions with sera from patients with Paragonimus westermani and Metagonimus yokogawai, while the 7 kDa antigen did not, suggesting that the 7 kDa antigen has high specificity. The 30 kDa antigen reacted with some past clonorchiasis sera, whereas the 7 kDa antigen did not, supporting that antibodies to the 7 kDa antigen are not present in sera from past clonorchiasis patients. In an endemic area, 92% (23/25) of active clonorchiasis patients and 91% (10/11) of mixed infection patients with C. sinensis and M. yokogawai had IgG antibodies to the 7 kDa antigen, while 40% (6/15) of past clonorchiasis individuals and 43% (3/7) of metagonimiasis patients cross-reacted to the antigen. These data suggest that the 7 kDa antigen in an excretory-secretory antigen may serve as a marker of an active clonorchiasis with reliable specificities in past clonorchiasis, paragonimiasis and metagonimiasis.

Figures


Fig. 1
Immunoblot analyses of CsW and CsE1-4 of C. sinensis probed with paired infection (I) and 6-month post-treatment (T) sera from praziquantel-cured (A) and praziquantel-failed (B) clonorchiasis patients. Amounts of 5 µg protein from each antigen were electrophoresed. Molecular masses in kDa were estimated with standard markers of Bio-Rad. Closed arrow-heads mean 30 and 7 kDa antigen bands that were observed remarkably; open ones mean these bands that were not discernible.


Fig. 2
Immunoblot analyses of CsW and CsE1-4 of C. sinensis reacted with paragonimiasis (A), metagonimiasis (B) and normal control sera (C).


Fig. 3
Immunoblot analyses of CsW and CsE1-4 of C. sinensis reacted with the past clonorchiasis sera (1-5) and conjugate control (6).


Fig. 4
Immunoblot analysis of CsE2 of C. sinensis reacted with paired infection (left strips) and 6-month post-treatment (right strips) sera from praziquantel-cured (A) and praziquantel-failed (B) clonorchiasis patients. Amount of 65 µg protein was electrophoresed in a preparative tract and 40 nitrocellulose strips were prepared.


Fig. 5
Immunoblot analysis of CsE2 of C. sinensis reacted with sera from past clonorchiasis (A), active clonorchiasis (B), metagonimiasis (C), and mixed clonorchiasis and metagonimiasis patients (D).

Tables


Table 1
Immunoblot analyses of 30 and 7 kDa antigens of CsE2 probed with paired infection and 6-month post-treatment sera from praziquantel-cured and praziquantel-failed clonorchiasis patients


Table 2
Immunoblot analysis of 7 kDa antigen of CsE2 probed with sera from various subjects in an endemic area of clonorchiasis and meta gonimiasis

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