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Korean J Parasitol > Volume 34(4):1996 > Article

Original Article
Korean J Parasitol. 1996 Dec;34(4):239-246. English.
Published online Dec 20, 1996.  http://dx.doi.org/10.3347/kjp.1996.34.4.239
Copyright © 1996 by The Korean Society for Parasitology
Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections
Neira de Dominiguez, and Alexis Rodriguez-Acosta*
Universidad Central de Venezuela Tropical Medicine Institute Immunochemistry Department Apartado 47423 - Caracas 1041 Venezuela.

FACULTAD DE FARMACIA-UCV

Received March 28, 1996; Accepted August 17, 1996.

Abstract

The usefulness of malaria diagnosis by Plasmodium falciparum-GDH (NADP+), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against GDH (NADP+) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. GDH (NADP+) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and anti-GDH (NADP+) of Proteus spp, recognized epitopes in Venezuelan isolates, and Colombian and Brasilian malarial strains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.

Figures


Fig. 1
GDH (NADP+) separation procedure from plasma of malaria patients by affinity chromatography.


Fig. 2
Determination of IgG anti-GDH (NADP+) in plasma of malaria patients by direct ELISA.


Fig. 3
Determination of anti-GDH (NADP+) IgG antibody in plasma of malaria patients by indirect ELISA


Fig. 4
Indirect ELISA evaluation of the GDH (NADP+) specificty, compared to other Plasmodium species.


Fig. 5
Indirect immunofluorescence assay of different preparations.

Tables


Table 1
Percentages of residual antigenicity of heat treated GDH (NADP+)


Table 2
Mean values (X ± S.D) and sensitivity (%) of GDH (NADP+) obtained in two ELISA methods

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