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Korean J Parasitol > Volume 34(4):1996 > Article

Original Article
Korean J Parasitol. 1996 Dec;34(4):239-246. English.
Published online Dec 20, 1996.  http://dx.doi.org/10.3347/kjp.1996.34.4.239
Copyright © 1996 by The Korean Society for Parasitology
Glutamate dehydrogenase antigen detection in Plasmodium falciparum infections
Neira de Dominiguez, and Alexis Rodriguez-Acosta*
Universidad Central de Venezuela Tropical Medicine Institute Immunochemistry Department Apartado 47423 - Caracas 1041 Venezuela.


Received March 28, 1996; Accepted August 17, 1996.


The usefulness of malaria diagnosis by Plasmodium falciparum-GDH (NADP+), obtained by affinity chromatography, is demonstrated in ELISA assays, testing IgG antibodies against GDH (NADP+) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. GDH (NADP+) thermal stability was demonstrated in a high heat resistance assay. The immunofluorescence assay demonstrated that anti-culture (P. falciparum) supernatant serum and anti-GDH (NADP+) of Proteus spp, recognized epitopes in Venezuelan isolates, and Colombian and Brasilian malarial strains. The antigen is soluble, with high specificity, is a potent immunogen and is thermoresistant.


Fig. 1
GDH (NADP+) separation procedure from plasma of malaria patients by affinity chromatography.

Fig. 2
Determination of IgG anti-GDH (NADP+) in plasma of malaria patients by direct ELISA.

Fig. 3
Determination of anti-GDH (NADP+) IgG antibody in plasma of malaria patients by indirect ELISA

Fig. 4
Indirect ELISA evaluation of the GDH (NADP+) specificty, compared to other Plasmodium species.

Fig. 5
Indirect immunofluorescence assay of different preparations.


Table 1
Percentages of residual antigenicity of heat treated GDH (NADP+)

Table 2
Mean values (X ± S.D) and sensitivity (%) of GDH (NADP+) obtained in two ELISA methods

1. Benacerraf B. Role of MHC gene products in immune regulation. Science 1981;212(4500):1229–1238.
2. Butterworth AE. Acta Trop 1987;44:31–40.
3. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8(9):871–874.
4. Heidrich HG, Mrema JE, Van der Jagt DL, Reyes P, Rieckmann KH. Isolation of intracellular parasites (Plasmodium falciparum) from culture using free-flow electrophoresis: separation of the free parasites according to stages. J Parasitol 1982;68(3):443–450.
5. Hempelmann E, et al. Protozoology 1982;29:637–642.
6. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
7. Ling IT, Cooksley S, Bates PA, Hempelmann E, Wilson RJ. Antibodies to the glutamate dehydrogenase of Plasmodium falciparum. Parasitology 1986;92(Pt 2):313–324.
8. Roth EF Jr, Schulman S, Vanderberg J, Olson J. Pathways for the reduction of oxidized glutathione in the Plasmodium falciparum-infected erythrocyte: can parasite enzymes replace host red cell glucose-6-phosphate dehydrogenase?. Blood 1986;67(3):827–830.
9. Sherman IW, Peterson I, Tanigoshi L, Ting IP. The glutamate dehydrogenase of Plasmodium lophurae (avian malaria). Exp Parasitol 1971;29(3):433–439.
11. Thaithong S, Beale GH, Fenton B, McBride J, Rosario V, Walker A, Walliker D. Clonal diversity in a single isolate of the malaria parasite Plasmodium falciparum. Trans R Soc Trop Med Hyg 1984;78(2):242–245.
13. Trager W, et al. Nature(London) 1978;237:621–622.
14. Van der Jagt DL, Intress C, Heidrich JE, Mrema JE, Rieckmann KH, Heidrich HG. Marker enzymes of Plasmodium falciparum and human erythrocytes as indicators of parasite purity. J Parasitol 1982;68(6):1068–1071.
15. Van der Jagt DL, Hunsaker LA, Kibirige M, Campos NM. NADPH production by the malarial parasite Plasmodium falciparum. Blood 1989;74(1):471–474.
16. Voller A, Bidwell D, Huldt G, Engvall E. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull World Health Organ 1974;51(2):209–211.
19. Wilson RJ, McGregor IA, Hall P, Williams K, Bartholomew R. Antigens associated with Plasmodium falciparum infections in man. Lancet 1969;2(7613):201–205.
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