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Korean J Parasitol > Volume 33(2):1995 > Article

Original Article
Korean J Parasitol. 1995 Jun;33(2):101-106. Korean.
Published online Jun 20, 1995.  http://dx.doi.org/10.3347/kjp.1995.33.2.101
Copyright © 1995 by The Korean Society for Parasitology
Serologic response of normal Korean children to Pneumocystis carinii as observed by immunoblot
Hyung Nam Moon,1Sung-Tae Hong,*2 and Soon-Hyung Lee2
1Department of Pediatrics, Ulsan University Asan Medical Center, Seoul 138-040, Korea.
2Department of Parasitology and Institute of Endemic Diseases, Seoul National University College of Medicine, Seoul 110-799, Korea.
Received May 08, 1995; Accepted May 17, 1995.

Abstract

Soluble protein of purified Pneumocystis carinii was prepared from experimentally infected rats. SDS-PAGE of the crude antigen resolved about 20 protein bands from 20 to 200 kDa. Out of them, 116 kDa band strongly reacted and 45-55 and 100 kDa bands reacted weakly to the positive reference human serum from U.S.A. Western blot analysis with sera of 130 normal children and 15 newborns in Korea revealed specific IgG antibody reaction to 40-55 and 116 kDa protein bands. Forty percent (40.0%) of the 145 sera were positive with any of the antigenic protein bands of P. carinii. The positive rate was 56% in 50 males and 33.3% in 48 females. The protein bands 40-55 and 116 kDa from rat P. carinii were confirmed to cross-react with human sera in Korea.

Figures


Fig. 1
Western blot pattern of P. carinii antigen to human sera. Lanes 1 & 6, size marker; 2, P. carinii crude antigen, Coomassie blue stained; 3, serum from a child positive by ELISA; 4, serum from a healthy adult male; 5, positive reference serum from USA.


Fig. 2
Western blot pattern of P. carinii crude antigen to human sera from Korea. 1, Coomassie blue stained bands; 2, size marker; 3, PBS reacted; 4, serum from a newborn infant; 5, serum from an ELISA positive adult; 6 & 7, serum from children; 8, serum from an adult with P. carinii pneumonia.

Tables


Table 1
Anti-P. carinii IgG antibody in serum fo normal Korean children as observed by Western blot

References
1. Blumenfeld W, Mandrell RE, Jarvis GA, Griffiss JM. Localization of host immunoglobulin G to the surface of Pneumocystis carinii. Infect Immun 1990;58(2):456–463.
 
2. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–254.
3. Garbe TR, Stringer JR. Molecular characterization of clustered variants of genes encoding major surface antigens of human Pneumocystis carinii. Infect Immun 1994;62(8):3092–3101.
 
4. Graves DC, McNabb SJ, Worley MA, Downs TD, Ivey MH. Analyses of rat Pneumocystis carinii antigens recognized by human and rat antibodies by using western immunoblotting. Infect Immun 1986;54(1):96–103.
 
5. Hong ST. Serologic response to Pneumocystis carinii of Seoul National University Hospital patients. Korean J Parasitol 1991;29(4):355–361.
 
6. Kovacs JA, Halpern JL, Swan JC, Moss J, Parrillo JE, Masur H. Identification of antigens and antibodies specific for Pneumocystis carinii. J Immunol 1988;140(6):2023–2031.
 
7. Masur H. Clinical studies of Pneumocystis carinii and relationships to AIDS. J Protozool 1989;36(1):70–74.
  
8. Pifer LL, Hughes WT, Stagno S, Woods D. Pneumocystis carinii infection: evidence for high prevalence in normal and immunosuppressed children. Pediatrics 1978;61(1):35–41.
 
9. Smulian AG, Stringer JR, Linke MJ, Walzer PD. Isolation and characterization of a recombinant antigen of Pneumocystis carinii. Infect Immun 1992;60(3):907–915.
 
10. Smulian AG, Sullivan DW, Linke MJ, Halsey NA, Quinn TC, MacPhail AP, Hernandez-Avila MA, Hong ST, Walzer PD. Geographic variation in the humoral response to Pneumocystis carinii. J Infect Dis 1993;167(5):1243–1247.
  
11. Stringer SL, Garbe T, Sunkin SM, Stringer JR. Genes encoding antigenic surface glycoproteins in Pneumocystis from humans. J Eukaryot Microbiol 1993;40(6):821–826.
  
12. Theus SA, Sullivan DW, Walzer PD, Smulian AG. Cellular responses to a 55-kilodalton recombinant Pneumocystis carinii antigen. Infect Immun 1994;62(8):3479–3484.
 
13. Walzer PD, Linke MJ. A comparison of the antigenic characteristics of rat and human Pneumocystis carinii by immunoblotting. J Immunol 1987;138(7):2257–2265.
 
14. Walzer PD, Stanforth D, Linke MJ, Cushion MT. Pneumocystis carinii: immunoblotting and immunofluorescent analyses of serum antibodies during experimental rat infection and recovery. Exp Parasitol 1987;63(3):319–328.
  
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