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Korean J Parasitol > Volume 31(3):1993 > Article

Original Article
Korean J Parasitol. 1993 Sep;31(3):223-230. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1993.31.3.223
Copyright © 1993 by The Korean Society for Parasitology
Passive transfer of immunity against Cryptosporidium infection in neonatal mice using monoclonal antibodies
Myung-Hwan Cho*
Department of Biology, College of Sciences, Kon-Kuk University, Seoul 133-701, Korea.
Received June 03, 1993; Accepted July 14, 1993.

Abstract

Monoclonal antibodies (mAb) against merozoites and sporozoites of the protozoan parasite Cryptosporidium parvum were examined for potential modulation of cryptosporidial infections in vivo by daily oral mAb administration to oocyst-inoculated neonatal mice. Monoclonal-treated neonatal mice were sacrificed four and eight days post infection (pi). Differences in infection rates were observed among the treatment groups at the p < 0.05 level. Suckling mice treated daily with orally administered mixtures of mAbs (ascitic fluids) showed significantly reduced parasite loads compared to control mice at four and eight days pi, while suckling mice receiving mAb Cmg-3 alone showed significant differences only at 4 days pi., suggesting that passive transfer of mAb may be of value in controlling cryptosporidial infections.

Figures


Fig. 1
Hematoxylin and eosin stained ileal sections from mice infected at 4 days of age and treated with saline (A) and monoclonal antibodies (B) until sacrificed 4 days later (8 days of age). Parasitic life cycle stages are present on the surface of the villous epithelium. A: × 100, B: × 400

Tables


Table 1
Experimental groups anti-crytosporidial monoclonal antibody used in passive transfer prophylaxis of BALB/c neonatal mice


Table 2
Cryptosporidial infection rates among mice in monoclonal antibody passive transfer prophylaxis groups


Table 3
Repeated measures analysis of variance of between and within subjects effects in anti-crytosporidial mAb passive transfer prophylaxis groups


Table 4
Multiple comparisons-differences between monoclonal antibldy passive rtansfer prophylaxis group means

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