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Korean J Parasitol > Volume 29(3):1991 > Article

Original Article
Korean J Parasitol. 1991 Sep;29(3):245-257. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.3.245
Copyright © 1991 by The Korean Society for Parasitology
Antigenic localities in the tissues of Metagonimus yokogawai observed by immunogoldlabeling method
H Ahn,H J Rim and S J Kim*
Department of Parasitology and Institute for Tropical Endemic Diseases, College of Medicine, Korea University, Seoul 136-701, Korea.
Abstract

In order to determine the antigenic localization in the tissues of the adult Metagonimus yokogawai, immunogoldlabeling method was applied using serum immunoglobulins(IgG) of cats which were infected with isolated metacercariae from Plecoglossus altivelis. The sectioned worm tissue was embedded in Lowicryl HM 20 medium and stained with infected serum IgG and protein A gold complex(particle size: 12 nm). It was observed by electron microscopy at each tissue of the worm.

The gold particles were observed on the tegumental syncytium as well as cytoplasm of tegumental cells and epithelial lamella of the caecum. The gold particles were not observed on the basal lamina of the tegument, interstitial matrix of the parenchyma, the muscle tissue and mitochondria of the tegument.

The gold particles were specifically labeled in the secretory granules in the vitelline cells. They were also labeled on the lumen of bladder and egg shell.

The above findings showed that antigenic materials in the tissue of adult worms were specifically concentrated on the tegumental syncytium as well as cytoplasm of tegumental cells and epithelial lamella of the caecum.

Figures


Figs. 1-3
Fig. 1. Electron micrographs of the tegument of the worm, which was reacted with cat IgG from noninfected control, showed the tegumental syncytium(TS), basal layer(BL), circular muscle(CM) layer, longitudinal muscle(LM) later, interstitial matrix(IM) and tegumental cell cytoplasm(TCC). Gold particles were not labeled on the tegument or other portions of the tissue. Bar=1 µm (×28,000)

Figs. 2, 3. The tegumental tissue of the worm reacted with specific antibody(IgG) from infected dog. Gold particles were specifically labeled in the tegumental syncytium and cytoplasm of the tegumental cell. Bar=1 µm (×17,000; ×28,000)



Figs. 4-6
Fig. 4. The vitelline gland of the worm which was reacted with cat IgG from noninfected control. Gold particles were not labeled on the cytop;asm and secretory granules of vitelline gland cell. Bar=1 µm (×28,000)

Figs. 5, 6. The vitelline gland of the worm which reacted with specific antibody(IgG) from infected cat. Gold particles were very specifically labeled on the secretory granules of vitelline gland cell cytoplasm. Bar=1 µm (×17,000; ×28,000)



Figs. 7-8
Fig. 7. The caecal section of the worm reacted with control group cat IgG from noninfected control. Gold particles were not labeled on the all area of the caecum. Bar=1 µm (×28,000)

Fig. 8. The caecal section of the worm reacted with specific antibody (IgG) from infected cat IgG. Gold particles were predominantly labeling on the caecum tegumental syncytium. Bar=1 µm (×28,000)



Figs. 9-11
Fig. 9. The sperms in the seminal receptacle of the worm which reacted with specific antibody(IgG) from infected cat. Gold particles were predominantly labeled on the tail part of the sperm and interstitial matrix of the seminal receptacle cell. Bar=1 µm (×28,000)

Fig. 10. The epithelium of excretory bladder of the worm reacted with control group dog IgG from noninfected control. Gold particles were predominantly labeled on the epithelial lamellae. Bar=1 µm (×17,000)

Fig. 11. The epithelium of excretory bladder of the worm which reacted with specific antibody(IgG) from infected cat. Gold particles were predominantly labeled on the epithelial lamellae. Bar=1 µm (×17,000)



Figs. 12-13
Fig. 12. The egg in the uterus of the worm reacted with control group cat IgG from noninfected control. Gold particles were not labeled on the egg shell. Bar=1 µm (×28,000)

Fig. 13. The egg in the uterus of the worm which reacted with specific antibody(IgG) from infected cat. Gold particles were predominantly labeled on the epithelial lamellae. Bar=1 µm (×28,000)


Tables


Table 1
Quantitative density of the labeled gold particles in the tissues of Metagonimus yokogawai reacted with antibody(IgG)* obtained from cats infected with Metagonimus yokogawai

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