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Korean J Parasitol > Volume 29(2):1991 > Article

Original Article
Korean J Parasitol. 1991 Jun;29(2):139-148. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1991.29.2.139
Copyright © 1991 by The Korean Society for Parasitology
Isolation and identification of Cryptosporidium from various animals in Korea. I. Prevalence of Cryptosporidium in various animals
J K Rhee,Y S Seu and B K Park
Department of Parasitology, School of Veterinary Medicine, Chonbuk National University, Chonju, Korea.

Cryptosporidium, a coccidian protozoa, commonly causes a self-limiting diarrheal illness in humans and animals. Fecal samples from various animals in Chonbuk district were observed using Sheather's flotation technique, Kinyoun's modified acid-fast staining, and osmic acid pre-fixed Giemsa staining. The oocysts were detected in 74 cages (29.6%) out of 250 cages of mature mice, 26 (13.3%) out of 195 mature house rats, 75(15.0%) out of 4-week-old 500 fowls, 98(19.9%) out of 6 to 8-month-old 500 pigs, and 111(22.2%) out of 2 to 5-year-old 500 dairy cattle, respectively. The degree of prevalence was slight in general, but actual prevalence was higher than infection rate because the detection rates were higher in repeated-preparation examinations in comparison to the first examination. Meanwhile, large and small types of oocysts were detected from mice, house rats, pigs, and cattle, and medium type from fowls.


Figs. 1-4
Fig. 1. Large (arrow) and small (triangle) types of Cryptosporidium oocysts concentrated from feces of infected mice by sugar centrifugal floatation method, ×670.

Fig. 2.Cryptosporidium oocysts concentrated from feces of infected chicken by sugar centrifugal floatation method, ×500.

Fig. 3. Large type of Cryptosporidium oocysts from mice, osmic acid pre-fixed Giemsa staining, ×1,675.

Fig. 4.Cryptosporidium oocysts from chicken, osmic acid pre-fixed Giemsa staining, ×1,675.

Figs. 5-8
Fig. 5. A HE-stained paraffin section of the stomach of a moue infected with large type of Cryptosporidium. Most gastric glands of the pars glandularis are filled with large numbers of the protozoa, ×1000.

Fig. 6. A HE-stained paraffin section of the bursa of Fabricius of a chick infected with Cryptosporidium. Cryptosporidia of various stages are attached to the surface of the lining epithelial cells, ×1600.

Fig. 7. A scanning electron photomicrograph showing the parasites in the pit of the gastric glands of a mouse infected with large type of Cryptosporidium, bar=10µm.

Fig. 8. A scanning electron photomicrograph showing large number of the parasites on the villous epithelial surface from the ileum of a cattle infected with Cryptosporidium, bar=10µm.


Table 1
Effect of repeated-preparation examinations on detection rates of Cryptosporidium oocysts in swine

Table 2
Prevalence of Cryptosporidium in mice

Table 3
Prevalence of Cryptosporidium in house rats

Table 4
Prevalence of Cryptosporidium in fowls

Table 5
Prevalence of Cryptosporidium in swine

Table 6
Prevalence of Cryptosporidium in dairy cattle

Table 7
Dimensions of Cryptosporidium oocysts in various animals (Kinyoun modified acid-fast staining preparations)

Table 8
The number of Cryptosporidium oocysts detected in feces

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