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Korean J Parasitol > Volume 28(1):1990 > Article

Original Article
Korean J Parasitol. 1990 Mar;28(1):53-62. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1990.28.1.53
Copyright © 1990 by The Korean Society for Parasitology
Recovery rate, growth and development of Heterophyopsis continua in experimental chicks
S J Hong,S H Lee,*J Y Chai,* and B S Seo*
Department of Parasitology, College of Medicine, Gyeongsang National University, Chinju 660-280, Korea.

The growth and developmental pattern of H. continua was observed after experimental infection of their metacercariae to chicks. The recovery rate of worms from the chicks at 1 to 28 days post-infection (PI) was 12.8% in average. The rate remained fairly high for early 4 days of infection but decreased thereafter rapidly till 28 days PI. Most of the flukes, 91.9%, were recovered from the ileum of the chicks. In metacercariae, genital organs such as the ovary, testes, seminal vesicle, seminal receptacle and genital sucker were recognizable. At one day PI Mehlis' gland appeared, and at 2 days follicular vitellaria were observed. At 3 days PI, eggs were formed in the uterine tubule and increased in number as the worm grew old. The worms reached 2,990 µm in length and 525 µm in width at 28 days PI. Genital organs developed rapidly in early stages of infection but slowly thereafter to 28 days PI, whereas non-genital organs developed steadily through the infection period. It was proved by this experiment that chicks should be a moderately suitable final host of H. continua.


Fig. 1
(A) Excysted metacercaria of H. continua. Developmental stages recovered from experimental chicks at 1 day (B), 2 days (C), 3 days (D), 4 days (E), 6 days (F), 8 days (G), 14 days (H) and 28 days (I) post-infection (PI). Figures A~I were enlarged at the same scale.

Fig. 2
The organogenesis of H. continua. (A) 'C'-shaped seminal vesicle (arrow heads), posterior to the genital sucker (GS), metacercaria. (B) Retort-form seminal receptacle (arrow heads), posterior to the ovary (OV), metacercaria. (C) Mehlis' gland (MG) appeared in front of ovary 1 day PI. (D) 2 days PI seminal vesicle (arrow heads) sacculated into right and left lobes at the middle part (arrow) and follicular vitellaria behind the seminal vesicle to the posterior end of the body. (E) uterine tubule (star) around the ovary and seminal receptale containing sperms at 2 days PI. (F) intra-uterine eggs at 3 days PI.

Fig. 3
Rodlets on the gonotyl of the genital sucker (GS), a 28-day-old worm.

Fig. 4
Growth curves of the genital (A) and nongenital (B) organs of H. continua recovered from the experimental chicks.


Table 1
Recovery of H. continua from the chicks experimentally infected with the metacercariae

Table 2
Measurements of H. continua recovered from the experimentally infected chicks

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