Passive transfer of immunity against <i>Cryptosporidium</i> infection in neonatal mice using monoclonal antibodies

, Cho, M H

doi:1993.31.3.223

Korean J Parasito > Volume 31(3):1993 > Article

Original Article


Korean J Parasitol. 1993 Sep;31(3):223-230. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1993.31.3.223
Copyright © 1993 by The Korean Society for Parasitology


Passive transfer of immunity against Cryptosporidium infection in neonatal mice using monoclonal antibodies
Myung-Hwan Cho^*
Department of Biology, College of Sciences, Kon-Kuk University, Seoul 133-701, Korea.

Received June 03, 1993; Accepted July 14, 1993.

Abstract
Monoclonal antibodies (mAb) against merozoites and sporozoites of the protozoan parasite Cryptosporidium parvum were examined for potential modulation of cryptosporidial infections in vivo by daily oral mAb administration to oocyst-inoculated neonatal mice. Monoclonal-treated neonatal mice were sacrificed four and eight days post infection (pi). Differences in infection rates were observed among the treatment groups at the p < 0.05 level. Suckling mice treated daily with orally administered mixtures of mAbs (ascitic fluids) showed significantly reduced parasite loads compared to control mice at four and eight days pi, while suckling mice receiving mAb Cmg-3 alone showed significant differences only at 4 days pi., suggesting that passive transfer of mAb may be of value in controlling cryptosporidial infections.

Figures


	Fig. 1 Hematoxylin and eosin stained ileal sections from mice infected at 4 days of age and treated with saline (A) and monoclonal antibodies (B) until sacrificed 4 days later (8 days of age). Parasitic life cycle stages are present on the surface of the villous epithelium. A: × 100, B: × 400

Tables


	Table 1 Experimental groups anti-crytosporidial monoclonal antibody used in passive transfer prophylaxis of BALB/c neonatal mice


	Table 2 Cryptosporidial infection rates among mice in monoclonal antibody passive transfer prophylaxis groups


	Table 3 Repeated measures analysis of variance of between and within subjects effects in anti-crytosporidial mAb passive transfer prophylaxis groups


	Table 4 Multiple comparisons-differences between monoclonal antibldy passive rtansfer prophylaxis group means

References


1.	Arrowood MJ, Mead JR, Mahrt JL, Sterling CR. Effects of immune colostrum and orally administered antisporozoite monoclonal antibodies on the outcome of Cryptosporidium parvum infections in neonatal mice. Infect Immun 1989;57(8):2283–2288.

2.	Campbell PN, Current WL. Demonstration of serum antibodies to Cryptosporidium sp. in normal and immunodeficient humans with confirmed infections. J Clin Microbiol 1983;18(1):165–169.

3.	Casemore DP. The antibody response to Cryptosporidium: development of a serological test and its use in a study of immunologically normal persons. J Infect 1987;14(2):125–134.

4.	Collier AC, Miller RA, Meyers JD. Cryptosporidiosis after marrow transplantation: person-to-person transmission and treatment with spiramycin. Ann Intern Med 1984;101(2):205–206.

5.	Current WL. Cryptosporidiosis. J Am Vet Med Assoc 1985;187(12):1334–1338.

6.	Current WL, Reese NC, Ernst JV, Bailey WS, Heyman MB, Weinstein WM. Human cryptosporidiosis in immunocompetent and immunodeficient persons. Studies of an outbreak and experimental transmission. N Engl J Med 1983;308(21):1252–1257.

7.	Davis PJ, Porter P. A mechanism for secretory IgA-mediated inhibition of the cell penetration and intracellular development of Eimeria tenella. Immunology 1979;36(3):471–477.

8.	Davis PJ, Parry SH, Porter P. The role of secretory IgA in anti-coccidial immunity in the chicken. Immunology 1978;34(5):879–888.

*11.*	Heine J, Moon HW, Woodmansee DB. Persistent Cryptosporidium infection in congenitally athymic (nude) mice. Infect Immun 1984;43(3):856–859.

*12.*	Lawn AM, Rose ME. Mucosal transport of Eimeria tenella in the cecum of the chicken. J Parasitol 1982;68(6):1117–1123.

*13.*	Letchworth GJ 3rd, Appleton JA. Passive protection of mice and sheep against bluetongue virus by a neutralizing monoclonal antibody. Infect Immun 1983;39(1):208–212.

*14.*	Mata L, Bolanos H, Pizarro D, Vives M. Cryptosporidiosis in children from some highland Costa Rican rural and urban areas. Am J Trop Med Hyg 984 Jan;33(1):24–29.

*15.*	Petersen C. Cellular biology of Cryptosporidium parvum. Parasitol Today 1993;9(3):87–91.

*16.*	Rose ME, Long PL, Bradley JW. Immune responses to infections with coccidia in chickens: gut hypersensitivity. Parasitology 1975;71(3):357–368.

*17.*	Sherman DM, Acres SD, Sadowski PL, Springer JA, Bray B, Raybould TJ, Muscoplat CC. Protection of calves against fatal enteric colibacillosis by orally administered Escherichia coli K99-specific monoclonal antibody. Infect Immun 1983;42(2):653–658.

*20.*	Whitmire WM, Kyle JE, Speer CA, Burgess DE. Inhibition of penetration of cultured cells by Eimeria bovis sporozoites by monoclonal immunoglobulin G antibodies against the parasite surface protein P20. Infect Immun 1988;56(10):2538–2543.